Cloning And Protein Expression Of Cucumber HPL Gene In E Coli

Cucumis sativus, is a widely cultivated plant in the gourd family (Cucurbitaceae).The cucumber is likely originated in India, where it appears to be cultivated for more than3,000years, and then spread to China. Cultivars of varied size and color are now grown in warm areas worldwide, have more strong and distinct flavors. It’s mild and sometimes "bland" flavor makes it a neutral addition to foods with strong flavors.Experiments were established when the HPL gene existence in cucumber with characteristics of aromatic substances nonadienal synthesis is closely related. Our laboratory has about120core cucumber germplasm resources resequenced. A part from these varieties known as the major aromatic substances nonadienal, the relative content of determination, were selected to study the differences in the main content of aromatic substances and apparently utilizing the data for future studies of the genetic differences of the different flavor varieties of cucumbers. Moreover, analysis of control of azelaic diene Aldehyde synthesis in two key enzymes namely hydro peroxide enzyme and hydrogen peroxide cleaving the expression of the enzymatic synthesis of each member of the gene family in cucumber, wherein part of the gene then cloned. In-vitro prokaryotic expression studies was carried out to identify the control of the cucumber major aromatic substances nonadienal synthesis genes, completely clear cucumber scent synthesis and metabolism of the molecular mechanism of the foundation. The experimental results obtained were clearly approved that the level of differences existed between cucumbers varieties in aromatic content was mainly due to their genetic differences. The wild cucumber aromatic substance content is much higher than the cultivated ones. Cultivars with low aromatic content tried to be hybridized with wild species to improve existing varieties where fragrance tasteless flavor quality is poor. Genetically, the HPL family genes expression in various organs of cucumber was studied and the amount of csa013551expression in various organs of cucumber is very low, not even the expression in the roots and fruits. Two other genes csa003557and csa003558in each organ, revealed a certain amount of expression, and the former is the rest of the various organs other than to uproot them, were higher than the latter in expression. We succeed in Cloning full-length coding sequence (cds) of HPL gene sequence into csa003558and successfully proteins induced the expression of each gene were explored, and HPL cas003558protein expression was induced in E. coli BL21.