Study On Uptake And Translocation Behavior Of Carabrone In Plant

Carabrone is the sesquiterpenoid lactone compound, which is widespread in theAsteraceae plants. Northwest A＆F University and Research and Development Centerof Biorational Pesticide researched the agricultural fungicidal activity of Carabronepreliminarily, cleared the inhibiting activity intensity and spectrum of Carabrone, andassessmented the prospects for the application of the compound. Therefore, thisarticle revealed the uptake and translocation behavior of Carabrone in wheat andcucumber systematically, so as to provide a basis for development and utilization ofcarabrone and its antifungal mechanisms research. The main results and conclusionswere given as follows:1Evaluate the uptake and translocation behavior of carabrone in plants by pottedtest.The control effects of Carbrone against2leaf diseases:wheat powdery mildewand cucumber powdery mildew, and2root diseases: wheat take-all and cucumberfusarium wilt was determined by potted test in spraying and irrigation application. Itwas found that:（1） In spraying application, the protective effect and the curativeeffect of Carabrone treating were76.63%and70.72%to Wheat powdery mildew,71.22%and75.62%to cucumber powdery mildew respectively at the concentrationof1000mg/L, but had no obviouse control effect on wheat take-all and cucumberfusarium wilt;（2） In irrigation application, carabrone showed noticeable control effecton whatever the leaf diseases or root diseases, the protective effect and the curativeeffect were70.23%and68.60%to wheat powdery mildew and70.98%and67.67%tocucumber powdery mildew at the concentration of1000mg/L. The protective effectand the curative effect to wheat take-all were78.71%and62.77%, and54.55%and45.74%to cucumber fusarium wilt at the concentration of1000mg/L.2. Evaluate the uptake and translocation behavior of carabrone in plants byHPLC.The uptake and translocation behavior of carabrone in wheat and cucumberseedling were further detected by high performance liquid chromatography （HPLC）.The result listed as follows：（1） The carabrone in soil could be uptake and translocatedupwards readily in wheat and cucumber seedling. The amount of carabrone absorpedand translocated upwards by the wheat and cucumber was9.4μg·g^-1and5.8μg·g^-14 hours after treatment, then gradually increase with extended of sampling time,reached the peak until60hours,64.7μg·g^-1for wheat and43.0μg·g^-1for cucumberrespectively, then begin to decreased to20.6μg·g^-1and11.5μg·g^-1respectively at96hours;（2） The carabrone in soil could be uptake and translocated upwards readily inwheat and cucumber seedling. The amount in stem and leaf of wheat cucumberreached77.9μg·g^-1and57.6μg·g^-1respectively at the first sampling time4h aftertreatment. The peak amount appeared at24hours,350.4μg·g^-1for wheat and309.7μg·g^-1for cucumber respectively, then began to decrease;（3） Uptake and downwardtranslocated activity of carabrone in wheat and cucumber plant after spraying them onleaf at the mass concentration of1000mg/L was not detected within72hours.