| Impatiens balsamina is a kind of annual herb plants from Impatiens balsaminaceae. Impatiensbalsamina is native to China and India etc. The Impatiens balsamina is widely distributed in China and itscommunity is rich. The Ornamental value and medical value of Impatiens balsamina are very important.But because of low efficiency and long period by the artificial cultivation, just depending on naturalresources can’t fulfill the requirement of medicine and modern society needs. The cultivation technologiesof plant tissue culture are considered to be a hopeful way which can control the growing condition of plants,accelerate the pace of propagation, and purify effectual element from plant cells. There are few reportsabout callus tissue culture on Impatiens balsamina. To establish a suitable plant regeneration system forgenetic improvement of Impatiens balsamina by plant tissue culture technology, we hope that we canaccumulate useful datas and information for tissue culture of Impatiens balsamina and themicropropagation system of the mutant plants by the study on the induction and differentiation of Impatiensbalsamina calus.We analysis the impact of different growth regulatory substance and other effect factors in the callusinduction and differentiation of Impatiens balsamina by the experiment results. A sterilization procedure forthe explants was developed in this study. The results showed that:1. The explants of young leaves could be most successfully sterilized by first70%alcohol for20s andthen by2%NaClO for30min. And the germination percentage was97%.2. Then we use young leaves from the aseptic seedings as explants of callus induction. The bestmedium of callus induction was MS+0.5mg·L-16-BA+1.0mg·L-12,4-D+0.2mg·L-1NAA,the induction ratewas100%.3. The best medium used to divide a large number of roots by callus differentiation was MS+0.2mg·L-1KT+0.05mg·L-1NAA+0.2mg·L-16-BA, The best medium used to divide buds by callusdifferentiation was MS+0.3mg·L-16-BA+0.2mg·L-1NAA.We also discuss the effect of different carbon source and pH and light condition in the callus inductionof Impatiens balsamina. The results indicated that:4. The inducing effect of carbon source level30g·L-1was the best, and then20g·L-1and40g·L-1. In the callus induction the induction rate of glucose was the most high of the three carbon sources, saccharosethe second, white granulated sugar the last. The callus quality induced by saccharose was much better thanthe others. As well as white granulated sugar could easily go brown. So we chose the level30g·L-1ofsaccharose as the best carbon source.5. The callus induction rate of the pH level5.8was84%, the best of all. The rate was56%of the pH4.8. But there were no callus induced by pH6.8.6. In the light culture,the callus quality induced was good, but in the dark culture, the callus inducedwas all dried. We chose light culture of1800lux/12h to induce the Impatiens balsamina callus. |
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