Genetic Diversity Of G. Tabacina And G. Tomentella From Part Of Southeast Coastal Areas In China

Glycine tabacina Benth grows on the hillside or barren grassland of the sea and coastal islands, and is distributed in Fujian (Meizhou Island, Dongshan Island), Australia, the Ryukyu Islands, the islands of the South Pacific, and Fiji. Glycine tabacina Benth has the advantages of disease resistance, heat and drought tolerance, so it has the potential application value in the improvement of cultivated soybean germplasm resources. Glycine tomentella Hayata is distributed in Australia, Papua New Guinea, Taiwan, Philippines and Fujian, Guangdong of Mainland China. So far, it has not been introduced by the artificial cultivation.The purpose of this study was to analyze the genetic diversity40from the southeast coastal areas in China by using ISSR markers, to explicate the of genetic variation of G. tabacina and G. tomentella accessions collected from different places at the molecular level, and further more to provide information for protection, development and utilization of G. tabacina and G. tomentella in southern coastal areas of China.In this study,20ISSR primers were used to analyze the genetic diversity of40Glycine tabacina accessions from Meizhou Island of Fujian province, and16G. tomentella accessions from Meizhou Island and Dongshan Island of Fujian province and Lufeng of Guangdong province. The results showed that:20ISSR primers could be used to amplify the genomic DNA of40G. tabacina accessions, and yielded a total of240bands, of which229bands (95.4%) were polymorphic. The average polymorphism information content (PIC) of20ISSR primers was0.27, ranging from0.03to0.37. The Dice similarity coefficient of the tested accessions ranged from0.3356to0.8639, and the average Dice coefficient was0.6753. This suggested that there was rich genetic diversity among the accessions of G. tabacina. Clustering results showed40accessions of G. tabacina were clustered into three major groups (Ⅰ, Ⅱ, Ⅲ), contained37accessions, group Ⅳ contained2accessions and group Ⅴ contained only1accession. Each major group contains one or more accessions from a different collection sites. This indicated that there existed a gene exchange among G. tabacina accessions from different collection sites.20ISSR primers could also be used to amplify the genomic DNA of40G. tabacina accessions, and yielded a total of122bands, of which52(42.62%) bands were polymorphic. The average polymorphism information content (PIC) of20ISSR primers was0.118. ranging from0.00to0.34. The Dice similarity coefficient ranged from0.8080to0.9908. the average Dice coefficient was0.9089. This shows that genetic diversity of G. tomentella accessions from Meizhou Island, Dongshan Island and Lufeng were relatively low. Clustering results showed that16accessions were divided into three groups, in which two groups contained only the accessions collected from Meizhou Island, the other group contains the accessions from different collection sites. This showed that there is little gene exchange among G. tomentella accessions from different collection sites.