| Mycotoxins are secondary metabolites produced by the fungus in the growth process, which are harmful to human and animals. Mycotoxins could affect our health through meat from livestock or poultry feeded contaminated grain and feed. The generation of mycotoxin cannot be completely avoided.So,it is siginificant to detect mycotoxins content in feed precisely, and to further attempt to degrade mycotoxins and release mycotoxins. Aflatoxin B1(AFB1) is a highly toxic substance and has been divided into Class I carcinogen by cancer research agency of WHO. Deoxynivalenol(DON) is the most seriously contaminanted fusarium toxins all over the world. Ochratoxin A (OTA) is highly toxic to the liver and kidney of animals, likely to be accumulated in livestock or poultry body,so as to cause harm to human through the food chain.OTA is the second most seriously mycotoxin that has caused worldwide economic losses after aflatoxin.The paper mainly study on the optimization and application of detection methods of fungal toxin include DON,OTA,AFB1by high performance liquid chromatography (HPLC) technology. These optimized HPLC technology for the research institutions and large enterprise groups feed the fast, efficient, accurate and economical detection of feed DON, OTA, AFB1toxins provide support and for the next feed detoxification, detoxification, transformation, mechanism provided technical support points.Test I The optimization of the determination of AFB1in feed using high performance liquid chromatography (HPLC) with fluorescence detectionHPLC equipped with fluorescence detection was optimized for detection of AFB1in feed.The extract and detection conditions have been optimized.The feed was tested by HPLC technology after purified by fluorescence detection. The coefficient of recovery was up to85～99%,and the relative standard deviation generally lower than3.50%. The limit of detection(LOD) was1.7ng·mL-1and the limit of quantity(LOQ) was5.67μg· kg-1.Test Ⅱ The optimization of the determination of DON in feed using high performance liquid chromatography (HPLC) with fluorescence detectionHPLC equipped with fluorescence detection was optimized for the detection of DON in feed.The extract and detection conditions have been optimized.The feed was tested by HPLC technology after purified by fluorescence detection. The coefficient of recovery was up to82-95%,and the relative standard deviation generally lower than3.68%. The limit of detection(LOD) was27ng· mL-1and the limit of quantity(LOQ) was90μg·kg-1.TestⅢThe optimization of the determination of OTA in feed using high performance liquid chromatography (HPLC) with fluorescence detectionHPLC equipped with fluorescence detection was optimized for detection of OTA in feed.The extract and detection conditions have been optimized.The feed was tested by HPLC technology after purified by fluorescence detection. The coefficient of recovery was up to85～96%,and the relative standard deviation generally lower than3.61%. The limit of detection(LOD) was0.038ng·mL-1and the limit of quantity(LOQ) was0.127μg·kg-1.TestIV Study on three fungaltoxins contamination in feedIn this study, using the optimized high performance liquid detection method,detecting content of AFB1,DON,OTA in270feed ingredients and300feed samples include formulated feed,concentrated feed,premix feed.Overall,AFB1,DON,OTA had a high detection rate in feed.DON was at a high level of the rate of exceeding the allowed standards,however,AFB1and OTA were relatively low.Feed detection proved that the optimized high performance liquid detection method is accurate. |
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