Screening Of Medicinal Mushrooms For Biological Conversion Of Kelp Waste And The Antioxidant Activity

In this paper, the medicinal mushrooms screening which were used inbiological conversion of kelp residue waste were on the basis of growth speed anddry weight yield. Using both the growth speed and antioxidant activity as the goalof optimizing parameters, the best fermentation conditions of excellent strainswere optimized by uniform design. Further more, the incubation time of the bestfermentation conditions was optimized using antioxidant activity as the goal ofoptimizing parameters. The efficient and high biological activity conditions ofbiological conversion of kelp residue waste using medicinal mushrooms werestudied. The researches above will provide a scientific basis for the biologicaltransforming kelp residue waste into high value-added industrial product ofmedicine, food and feed.With the studies of medicinal mushrooms screening from biologicalconversion of kelp residue waste, using the growth spee d as the major goal ofoptimizing parameters, and considered the dry weight yield at the same time,BOE6, SUB44,42and PHI30were selected for the optimization of fermentationconditions. The antioxidant activity of the solid fermentation product powder w asconfirmed preliminary.The optimization results of culture conditions indicated the best fermentationconditions for42: the optimal water content was70%, the initial medium pHvalue was5, wheat bran content was1%, glucose content was0.16%, and theoptimal culture temperature was25.9℃. The growth speed was1.5times as thespeed of before optimization. The antioxidant activity of the crude polysaccharideand alcohol precipitation supernatant of the excellent test groups was studied. Theresults indicated the antioxidant activity of th e crude polysaccharide was higherthan that of the alcohol precipitation supernatant, and the antioxidant activity of42was higher than that of BOE6and SUB44, besides the crude polysaccharidecontent of42was higher than that of BOE6and SUB44.The incubation time of the best solid fermentation conditions of42and BOE6were optimized, and the antioxidant activity was studied, which of the solidfermentation product powder, the crude polysaccharide, the alcohol precipitationsupernatant and the whole extracts. The results indicated that the crudepolysaccharide had the highest antioxidant activity. Using the antioxidant activityof the crude polysaccharide as the goal of the incubation time optimization, theoptimal incubation time of42was20days after overgrew, and the optimalincubation time of BOE6was5days after overgrew. The total antioxidant capacity of the crude polysaccharide extracted from both42and BOE6was3.9times asthat of the excellent group of the uniform design. It indicated that t he antioxidantcapacity got a better improved through the optimization. The total antioxidantcapacity of the crude polysaccharide extracted from42was2.2times as that of thecontrol, and the antioxidant capacity of the crude polysaccharide extracted fr omBOE6was2.7times as that of the control.Compared with the antioxidant activity of the positive control standard, theactivity of the test samples was higher than some of the positive control, itindicated that the antioxidant results were scientific and reliable, and the productof the biological conversion had high useful value. The biological conversion ofkelp residue waste is a practical and environmental friendly resource recyclingmethod.