Isolation And Identification Of Nicosulfuron Degrading Strain And Analysis Of Metabolomics

The isolation and identification of pesticide degrading microorganism is a basic biological repairwork in the environment. An enrichment culture technique was used to isolate nicosulfuron degradingbacteria from the soil of the field contaminated by pesticides.5.0g of the soil was added into100mLenrichment medium that contained100mg/L nicosulfuron.10mL of the culture was transferred weeklyinto fresh enrichment medium with increasing concentrations of Nicosulfuron from100to700mg/Land incubated under the same condition. Pure isolate was obtained by plating the final enrichmentmedium plate containing700mg/L nicosulfuron, and incubated. A nicosulfuron degrading strainYC-WZ1is isolated and identified. The YC-WZ1can fully degradate100mg/L nicosulfuron in6days.To learn more about the mechanisms of degradation in nicosulfuron degrading strain YC-WZ1,research methods of metabolomics were used. All the metabolites of the YC-WZ1in the presence ofnicosulfuron were detected. The samples were separated into the treatment group (TG) and the controlgroup (CG).After the derivation treatment of the samples. The Metabolomics’ data of the samples wasgot after the GC/TOF-MS analysis of the strain. GC/TOF-MS data of the samples from the two groupsTG and CG were analyzed by principal component analysis (PCA) to envisage the perturbation of themetabolic network of the nicosulfuron treatement strain. Projection to latent structure-discriminantanalysis (PLS) and orthogonal projection to latent structure-discriminant analysis (O-PLSDA) werecarried out on the integrated GC-TOF-MS datas allowing the identification of differential metabolites ofthe cell. And based on the data obtained, we can presume metabolic pathways of nicosulfuron degradingstrain YC-WZ1.The metabolomics data of the nicosulfuron degrading strain YC-WZ1suggested that the degradationof nicosulfuronis actually hydrolysis. The declining amount of amino acids in the strain cell indicatedthat the metabolism of amino acids provides necessary nitrogen nutrient of the cell. The increasingamount of the intermediate of carbohydrate metabolic pathway also suggests internal increasing demandfor energy of the TG group strains. Under the pressure of pesticide, the strain also produced a resistancematerial trehalose inside to protect the cell from toxic chemical substances. It also explain that themechanism of isolated strains survive under the condition of high concentration of nicosulfuron.By the use of metabolomics, we can get a more comprehensive, more stable overall metabolismpathway metabolism in microorganism. Metabolites changing in microbial cells under pesticideconditions can be used to infer changes in microbial adaptation to environmental stress associated withmetabolic pathways. This study offers a new perspective in the metabolomics of environmentalmicrobiology.