Preparation And Performance Of COL-based Implantable Artificial Hair

In recent years, a great number of people have alopecia due to various reasons, incidences of disease increase every year, and it tend to be higher in the young generation. Alopecia affects people’s normal work and life seriously. Implantable artificial hair become a new choice for the treatment of bald hair for people while many traditional treatments, such as drug treatment and autologous transplantation, can not meet the requirements of patients.Polyester and Collagen were used to synthesize COL/PET composite material by liquid graft polymerization method. Since PET has good biostability and reasonable biocompatibility and COL has Low antigenicity, preferably biocompatibility, and biodegradation. COL can enhance polyester monofilament cytocompatibility to meet the need of artificial hair implantation, which can be a new approach for alopecia.In this project COL which have good biocompatibility grafted onto the surface of PET monofilament by chemical graft polymerization. We studied the effects of main factors including the concentration of collagen solution and glutaraldehyde solution content on the graft quantity of COL/PET monofilament.The results showed that when the concentration of collagen solution was l.Omg/ml and the concentration of glutaraldehyde was1.0%, collagen in the surface of the COL/PET monofilament had the best graft quantity. SEM、FTIR、XPS test methods were used to investigate the morphological structure, molecular structure elements of COL/PET monofilament. Meanwhile, the weight and mechanical properties of monofilaments after AAC treatment and COL graft polymerization were analyzed. And combine capability between Collagen and PET monofilament, biostability and biocompatibility were also tested.The structures of properties of the COL/PET were studied. After COL graft polymerization, the weight gain rate of monofilament was0.36%and the weight gain rate increased with the concentration increasing of collagen solution. Meanwhile, SEM test showed that after COL graft polymerization, COL coated the surface of PET monofilament, filament surface became uneven and formed a continuous dense collagen membrane. FTIR test showed that the surface of PET monofilament was covered by COL and the helical structure of collagen macromolecules was not broken after graft polymerization, COL grafted in the filament surface kept protein activity. XPS showed that there was nitrogen’s characteristic absorption peak after COL graft polymerization, which indicated that COL was grafted on the surface of PET monofilament. It is consistent with FTIR. Mechanical properties test showed that the mechanical properties of monofilament had been decreased after AAC treatment, but they were increased after COL graft polymerization. The combine capability between PET monofilament and COL test showed that COL/PET monofilament had good degradation resistance. Biological stability test showed that COL/PET monofilament had good biostability in enzymatic papain solution and phosphate buffer solution, and the biostability in phosphate buffer solution was better than that of enzymatic papain solution. Cytotoxicity test showed that the COL/PET monofilament was not Cytotoxic and the cytocompatibility of PET monofilament had been improved after COL graft polymerization.