Preparation And Application Of Phosphorescence Silica Nanoparticle Probes

Phosphorescent heavy-metal complexes possess excellent photophysical properties, However, the majority of metal complex imaging agent due to the hydrophobic and toxicity is difficult to enter the cell biological optical function, therefore, it is necessary to select the appropriate nano-carrier. For the unique properties of adjustable pore diameter, uniform particle size, good dispersibility, compatibility, good biocompatibility and non-toxicity, surface also can be further functionalized and transparent optical, these features make the nanoparticles in photodynamic therapy, biological marker, detection and cell targeting. Biomedical fields have broad application prospects, has attracted more and more attention.In this paper, two kinds of phosphorescent complexes were designed and synthesized, and the metal complexes into silica nanoparticles, preparation of organic/inorganic hybrid silica nanoparticles (PMSN) phosphor. Cell experiments show that, PMSN has a strong phosphorescence emission, can rapidly penetrate the cell membrane into the cell, dyeing region is mainly in the cytoplasm.In addition to taking folic acid as targeting agents, a cancer cell targeting function of phosphorescent nanoparticles were prepared, cell experiments show that, with the cell membrane penetrability and cytoplasmic staining of good performance, but also has very significant to target cancer cells function.Based on the synthesis of organic/inorganic hybrid phosphorescent silica nanoparticles, we prepared silica nanoparticles with core-shell structure of phosphorescence probe, the probe comprises a solid phosphorescent silica nanoparticles and mesoporous silica layer coated on its surface. Transmission electron microscopy data show, the nanoparticles were well dispersed in water, uniform particle size, size of about100nm. Cell culture experiments, the probe has a strong phosphorescence emission, can rapidly penetrate the cell membrane into the cell, staining occurred mainly in the cytoplasmic region. After the phosphorescence of silica nanoparticles with core-shell structure shell adsorption on the platinum porphyrin complexes, found that the probe has a very good detection effect of oxygen, in the realization of the rapid detection and ratio method to measure the oxygen in the water, and the probe can be used repeatedly.