Preparation And Application Of Thermosensitivity Glycopolymers Nanofibers

Nowadays, according to the nanometer dimensions and rapid temperature responsiveness, intelligent polymer nanofibers as novel polymer materials have attracted growing attentions in biomedical material field in recent years. And at the same time, the glycosyl can improve the polymer hydrophilia and the biocompatibility characteristics et al., so glycosyl-based thermoresponsive polymeric materials thermoresponsive properties and their application bring to the forefront of researchers from all over the world.On the basis of numerous published research work, we designed and synthesized a series of novel sugar derivatives-based thermoresponsive polymeric materials, then prepared them into nanofibers and nanopaticles using the electrospinning method, and investigated their thermoresponsive properties and in vitro nonspecific proteins adsorption and specific adsorption in detail and also the materials biocompatibility quality. The main contents are following:Chapter1presents a detailed review of recent progress in glycosyl-based thermoresponsive polymeric materials, and the background of PNIPAM-based polymeric and the glycosyl-based nanofibers and nanoparticls.Chapter2presents the synthesis of the glycosyl-based thermoresponsive polymeric nanofibers. In order to research the different effects of different materials on the recognition performance, we use enzymatic synthesis method to prepare the6-O-vinyladipoyl-D-glucose (OVDG),6-O-vinylazelaicoyl-D-glucose (OVZG) and6-O-vinylsebacoyl-D-glucose (OVEG).A series of glycosyl-based thermoresponsive polymeric materials such as Poly (N-isopropylacryamide-co-6-O-vinyladipoyl-D-glucose)(Poly(NIPAM-co-OVDG)), Poly (N-isopropylacryamide-co-6-O-vinylazelaicoyI-D-glucose)(Poly(NIPAM-co-OVZG)), Poly(N-isopropylacryamide-co-6-O-vinylsebacoyl-D-glucose)(Poly(NIPAM-co-OVEG)) and were fabricated using a chemoenzymatic process and radical copolymerization. The structures of the glycopolymers were confirmed by1H NMR and FI-TR, and also the lower critical solution temperature (LCST) used by Ultraviolet visible light absorption. And the UV-vis data showed that the materials exhibited reversible temperature-responsive behavior, by alternating the monomer feed ratio, copolymers were synthesised to have their own distinctive lower critical solution temperature (LCST). Because of the glucosyl groups specific effects on the Concanavalin (Con A), it has the application on protein purification.The preparation of nanofibers of Poly(NIPAM-co-OVDG). Poly(NIPAM-co-OVZG)> Poly(NIPAM-co-OVEG) and PLCL using the electrospinning method. Adding PLCL into the fibers can deduce the solubleness of these fibers. From the results of SEM, we can see that the electrospinning is a very good method to prepare the fibers and the fibers diameters are nearly among200-500nm.Chapter3presents the research of the series of membranes static adsorption and the anti-specific adsorption at the different temperature. Take the Poly(NIPAM-co-OVDG) as the example to discuss the static adsorption of the unspecific proteins and the specific protein. The results indicate that when the Con A content is0.563mg/mL, the adsorption content is higher at25℃than at37℃. At the same time, we find that the Con A adsorption content can increase as the content of sugar content increasing.And also we choose the Poly(NIPAM-co-OVDG) as the example to discuss the adsorption event of nonspecific protein at different temperature and different type of membrane. We can see that at the same BSA content, the BSA adsorption content is lower at the37℃than at25℃.In chapter4, we choose the FITC-BSA and RBITC-BSA as the materials and discuss the specific proteins adsorption and desorption, and also the effect of temperature in these process. The results are measured by Confocal and fluorospectro photometer. The results indicate that these membrane can adsorption FITC-Con A, but nearly non-adsorption of RBITC-BSA. And aslo we take the Poly(NIPAAm-co-OVDG) as the experiment object to discuss the effect of temperature, and the result is that the FITC-Con A adsorption strength is higher at25℃than at37℃.In chapter5, we study the cytotoxicity of different materials using MTT method. The results indicate that the membrane have very friendly biocompatibility characteristic. We discuss the effect of Con Aon the cell proliferation further. We can see that adsorbed Con A membrane can kill the HeLa cells and also it can give us some suggestion in the apply field of target local release of drugs.In the chapter6, we summary the main content in this project, and also look into the future in this field.