Potential Capable For Reducing Heavy Metals In Sewage Sludge Biosurfactant Producer Bactium Isolated And Identificated

The isolated two stains bacterium use CTAB methylene blue agar media to screen for the ability to produce biosurfactant. Identification of bacteria use physiology and biochemistry characterize and16S rDNA molecular analysis. The biosurfactant purification by acid precipitate and organic solvent extracts. The purity product had been identity biochemical characterizations by FT-IR. The strain B1and B2was Proteus mirabilis and Pseudomonas aeruginosa, respectively. The B1can produce1.0g/L biosurfactant which was identified to lipopeptid. The B2can produce2.9g/L biosurfactant which was identified to rhamnolipid and can produce more biosurfactant to4.7g/L which use oil as solo carbon source. And the biosurfactant produced by B2have good emulsification ability. Sludge washing experiments were run in24h at200r/min25℃. The biosurfactant solution as the flushing solution to remove heavy metals from sewage sludge, the ratio of sludge mass to volume of washing solution is m/V=0.6/20(w/v).This lipopeptid and rhamnolopid all have good performance to reduce heavy metal from sewage sludge, the lipopeptid have higher removal rate that reduce efficiency of Cu and Zn was67.4%,44.5%at concentration of0.15%, respectively. At the same condition, rhamnolipid solution at0.5%can reduce63.7%,15.3%of Cu, Zn from sludge, respectively. The strain B1and B2isolation from the tannery sludge which contained heavy metal concentration, besides, the results show the bacteria B1, B2can tolerant heavy metal. The biosurfactant produce by B1, B2have high removal rate of heavy metals reduce from sewage sludge. The further research can with the emphasis on stain improvement and fermentation optimization.