Study On Spectral Property And Analytical Method Of Fluorescent Component Of Chinese Herbal Medicine Liangmianzhen

Fluorescent properties of Chinese herbal medicine Radix Zanthoxyli and its activecomponents, nitidine chloride, ethoxychelerythrine and toddalolatone, were studied in thisthesis. Influences of experimental conditions on fluorescence spectra were investigated.Fluorescence quantum yields and molar fluorescence coefficients of these components weremeasured. TLC scanning method and HPLC method with fluorescence detector for thedetermination of these components in Radix Zanthoxyli medicinal material were established.Four parts were included in this thesis:1. Fluorescent property of nitidine chloride was studied. It was found that acidity andorderly medium have appreciable impact on fluorescence spectrum. In aqueous solution withpH11.66, containing2.43mg mL^-1β-CD and10%methanol, fluorescence quantum yield ofnitidine chloride was measured to be0.12; molar fluorescence coefficient was1.92×10⁸Lmol^-1, measured at wavelength λ_ex/λ_em=277/413nm. A TLC-absorption scanning method anda TLC-fluorescence scanning method were proposed for the determination of nitidine chloridein a Radix Zanthoxyli sample, the results were0.097%and0.092%, respectively.2. Fluorescence property of ethoxychelerythrine was studied. In aqueous solution withpH11.51, containing10%methanol, fluorescence quantum yield of ethoxychelerythrine wasmeasured to be0.18. In aqueous solution with pH11.51, containing30%methanol, molarfluorescence coefficient was measured to be6.3×10⁷L mol^-1at wavelength λ_ex/λ_em=279/40⁸nm. A TLC-absorption scanning method and a TLC-fluorescence scanning method wereproposed for the determination of ethoxychelerythrine in the Radix Zanthoxyli sample, andthe results were0.098%and0.11%, respectively. Experiment revealed that TLC-fluorescencescanning method has higher sensitivity. Contents of nitidine chloride and ethoxychelerythrinein Radix Zanthoxyli were simultaneously determined by HPLC method with fluorescencedetector, the results were0.094%and0.0⁸4%, respectively.3. Fluorescence property of toddalolatone was studied. In neutral aqueous solutioncontaining10%methanol, fluorescence quantum yield of toddalolatone was measured to be0.02, and molar fluorescence coefficient was7.0×10⁶L mol^-1at wavelength λ_ex/λ_em=328/400nm. Using TLC-absorption scanning method, TLC-fluorescence scanning method and HPLCmethod with fluorescence detector, the content of toddalolatone in Radix Zanthoxyli samplewas determined to be0.62%,0.65%and0.64%, respectively.4. Fluorescence spectra of Radix Zanthoxyli at different conditions were studied. Theresults showed that there are many fluorescent components in Radix Zanthoxyli. Since theoverlapping of fluorescence spectra, contents of components can not be determined byfluorimetric method without pre-separation.