The Process Research Of Huaiqing Yam By Enzymatic Hydrolysis And Liquefied

In this paper, the process conditions of the enzymatic hydrolysis of fresh HuaiqingYam and the enzymatic kinetics of α-amylase hydrolyzed under medium-temperaturewere investigated. And then, the anti-oxidative of the huaiqing yam extractive wasexamined by the different methods in vitro oxidation experiment. The main contents andresults are as following:1. The process conditions of fresh Huaiqing Yam by enzymatic hydrolysis. Thebest enzymatic hydrolysis technological conditions of Cellulase, Pectinase and Papainenzyme are got by the single factor experiment and the orthogonal experiment, and onthis basis to get the best enzymatic hydrolysis technological conditions aftercompositing of these: in the materials/liquid for1:20, the proportion of Cellulaseadded amount0.075mg/mL, Pectinase added amount0.040mg/mL and Papain enzymeadded amount0.050mg/mL, the temperature for60℃, the pH value for4, the time for150min and. Then the optimum technological conditions of medium-temperatureα-amylase hydrolyzed Huaiqing Yam residues are got: the PH value for7, thetemperature for80℃, the time for20min and the enzyme added amount for0.060mg/mL.2. The technology conditions that the ultrasonic extracts fresh huaiqing yam andenzyme liquefaction after ultrasonic extraction. The best technological conditions ofultrasonic extraction are got by the single factor experiment and the orthogonalexperiment: the frequency for40kHz, the temperature for50℃, the time for30min, thematerial/liquid ratio for1:20. Then the optimum technological conditions ofmedium-temperature α-amylase hydrolyzed Huaiqing Yam residues are got: thetemperature for80℃, the PH value for8, the time for15min and the enzyme addedamount for0.050mg/mL.3. The kinetics of medium-temperature α-amylase hydrolyzed Huaiqing Yam. Inthe condition of the temperature for70℃, the pH for7and enzyme concentration for0.200mg/mL, the Michealis constant (Km) for6.6641mg/mL and maximum rate (Vm)for3.1588mg/mL·min. The kinetic model, including the factors such as substrateconcentration, enzyme concentration and temperature, was suit for the hydrolysis reaction under the temperature range from40~70℃.4. By using different extraction methods to get Huaiqing yam extractive of invitrooxidation experiment. To extractive of Huaiqing yam oxidation stability is studied.ultrasonic extractive has good oxidation resistance, composite enzyme extractive better,distilled water extractive antioxidant ability is bad.