Microsatellite Analysis About Genetic Diversity Of Giant Panda In Liangshan And Qinling Mountains

Giant panda is a rare and endemic animal in china, which only distributed in Qinling, Qionglai, Liangshan, Minshan, Daxiangling and Xiaoxiangling mountains at present. The habitat loss and fragmentation effecting about giant panda has aroused widespread concern in the world. it is necessary to konw the genetic background of the species in order to make effective conservation and management strategies. In this study, we use12microsatellite loci as molecular markers to analyze and discuses the genetic diversity and population history of giant pandas, in order to provide scientific basis for the protection of giant pandas.We collected90fecal samples from Qinling Mountains and59fecal samples from Liangshan Mountains in2012. The Qinling90fecal samples of DNA are from50giant pandas, the Liangshan59fecal samples are from36giant panda. SRY gene sex identification primers study Indicate that there are10female giant panda and23male giant panda in Qinling Mountains, there are10female giant panda and14male giant panda in Liangshan Mountains.In Liangshan Mountains, the average allele number was4.2500, the average observed heterozygosity is0.6250and the average expected heterozygosity is0.6110, the average PIC is0.5525. In Qinling Mountains, the average allele number was4.417, the average observed heterozygosity is0.4510and the average expected heterozygosity is0.4390, the average PIC is0.5525.K test showed that the Ame-u11, Ame-24、and AY161195three loci are obviously unimodal distribution, it infer that the giant panda population may have experienced population expansion in history.Bottleneck heterozygosity test and model test results show that giant pandas in Qinling Mountains have not experienced population bottlenecks, but giant pandas in Liangshan mountains may have experienced population bottlenecks.