Mechanisms Of Cold-acclimated Freezing Tolerance Of Choripora Bungeana

The normal growth of plants need a suitable temperature environment, plants grow in the below a certain temperature will damage and even death. So explored the frost resistance ability of plants have important biological significance. Chorispora bungeana is a perennial herbaceous plant which is growing at high altitudes and low temperature region. In recent years, this plants research more and more attention. For it to grow in harsh areas, and has a strong frost resistance research and increasing. Study found that most of the plants of frost resistance can be enhanced by cold acclimation, but cold acclimation to Chorispora bungeana the ability enhancement is not obvious. Cold acclimation mechanism of Chorispora bungeana has become a new part of the study of plant frost resistance.Based on Chorispora bungeana callus as experimental material, adopted4℃cold acclimatization and22℃cold acclimatization callus in dealing with the different conditions, research on the alpine cold acclimation, the effects of ion mustard antifreeze. Chorispora bungeana cold acclimation of callus with-4℃cold stress after24hours showed that higher electrical conductivity determination than non-acclimation of callus. Determination of proline and P5CS1gene expression, cold acclimation Chorispora bungeana of callus put up proline content is higher than the non-acclimation of callus, and P5CS1gene expression in the cold and there is no big difference between before and after acclimation. Callus of sugar content, found that after cold acclimation of sucrose and fructose content is higher than the non-acclimation of callus. In order to explore Chorispora bungeana callus of CBF pathway molecules expressing mechanism, we selected the CBF1gene expression analysis. The results showed that cold acclimation and non-acclimation in callus CBF1expression of no obvious distinguish, but the downstream CBF gene C0R15a and C0R78gene expression in the cold acclimation of callus is significantly higher than the non-acclimation expression of callus.CB12798gene is cold reply way of Chorispora bungeana in a antifreeze gene transcription factors, in order to understand the gene function, we also make a CB12798into Arabidopsis thaliana, observe its phenotype, and then uses the LT50lethal method whether transgenic Arabidopsis thaliana have cold resistance.Results show that the transgenic CB12798Arabidopsis LT50of before and after cold acclimation temperature is lower than wild-type Arabidopsis. In Arabidopsis thaliana CB12798still have played an important role in the ability of antifreeze This paper to explore Chorispora bungeana callus tested the non-acclimaiton and the cold acclimation conditions cold response analysis of molecular expression quantity, also analyzed the non-acclimation and the cold acclimation of proline and conductivity cell activity and organize the difference of the physical expression level such as sugar content, verified the regulation by the cold stress response gene expression in the Chorispora bungeana callus, the mechanism of cold tolerance in plants provides the information function, and the Chorispora bungeana cold resistant frozen mechanism provides a new research direction of reference.