Analysis Of Microbial Community Structure On Etone River Bottom Sludge

Etone River is the secondary tributary of Songhua River, the only river runningthrough ChanChun. New City reservoir, located in middle reaches of Etone River, isthe significant water source of ChanChun’s industry and agriculture. After Etone Riverflowing through ChanChun, all kinds of life sewage and industrial waste water arerunning into the river. The river severely contaminates, becoming black and olid allthe year and influencing the ecological landscape and residents’ health and lifeseriously. In addition, with the deterioration of river water quality, microbial safety ofriver water and sludge is reducing. Therefore, study microbial component is of greatsignificance for Etone’s biological monitoring and bioremediation.Because there are many uncultured microorganisms in the river sediment habitat,if just using traditional cultured techniques, the diversity of microbial communitystructure can not be studyed in-depth and comprehensively. However molecularbiology technology opens a new path for this study. To explore microbial componentof polluted river, this paper uses PCR–DGGE technology to analyze sediment bacteriaand fungi community structuret of Etone River.This study explores the total DNA extraction method of sediment samples first.Results indicated that using the improved soil extraction kit can obtain the bettersediment microbial DNA and can conduct subsequent PCR successfully.We collected upriver, middle-river and downriver sediment samples of EtoneRiver at different sites. After DNA extracted, the DNA is used as template to amplify16S rDNA V6-V8fragments with bacteria universal primer pair968F-GC-clamp/1401R, and at the same time to amplify18S rDNA V1-V2fragmentswith fungi universal primer pair NS1/Fung. Then proceed DGGE. Analysis of bacterial DGGE finger-print indicates that richness of bacteria in Etone is relativelyhigh. One third bacteria are useful for sewage purification. The main bacteriacommunity is Firmicutes. Richness of fungi in Etone was relatively low, and almosthalf of the fungi are useful for sewage purification. The main fungi community isAscomycota in7kinds of samples.This study firstly uses uncultured PCR-DGGE technology, analysing thesediment microbial community structure systematically, and parsing the classificationand belonging of sediment microorganisms which have purified potential. It is guidingsignificant to treat Etone synthetically and establishes important foundation to getuseful microorganisms in pollutional environment.