The Taxonomic Study Of Ectobiidae Based On DNA Barcoding

This thesis mainly deals with the taxonomic study of Ectobiidae based on DNA barcoding. The taxonomic status, morphology and problems of Ectobiidae are reviewed. The application in classification of insect, superiority and problems of DNA barcoding are reviewed.The genomic DNA was extracted from Ectobiidae cockroaches (including95male samples from41species,18genera and including4new species:Margattea angusta sp. nov., Margattea spinosa sp.nov., Episymploce conspicua sp.nov., Scalida normalis sp. nov., and unidentified15female and10nymph samples), which were stored in95%ethanol. Standard PCR amplification was used to sequence the COI fragment. Primers used for PCR in this study were LCO1490and HCO2198. Amplified DNA was658bp in length, excluding the primer regions. We compared barcode tree and genetic distance using DNA sequencing with morphological data. The results indicate that the differentiation time and degree are different among genera. Meanwhile, DNA barcoding identification techniques based on COI gene is feasible for Ectobiidae cockroaches, and the females and nymphs can be identified by COI-based DNA barcode with abundant male adults. Moreover, Pseudothyrsocera sinensis should be transferred to Episymploce according to the result.