| Abiotic stresses are main factors affecting plant growth and crop production. As model plant for molecular biology research, Arabidopsis has plenty of T-DNA insertion mutants and many other advantages for gene function characterization. In this research, highly abiotic stress responsive rice genes were identified from microarray data and their homologous genes in Arabidopsis were obtained by BLAST the DNA database. The gene TEM1with T-DNA insertion mutants were chosen for functional analysis in this research. T-DNA insertion mutants were obtained from the Arabidopsis mutant resources and overexpression transgenic Arabidopsis plants were obtained by Agrobacterium tumefaciens mediated transformation of our constructed overexpression vector.Under salt and drought stress treatment, the TEM1mutant plants were less affected to the damage of abiotic stresses than the WT and overexpression plants. Physiological and biochemical analysis showed that under the abiotic stresses, all of the TEM1mutants, overexpression plants and WT plants had decreased cell membrane stability, increased content of MDA and free proline. But the mutants showed less decreased cell membrane stability and less increased content of MDA compared to the overexpression and WT plants. While the mutants showed more increase content of free proline than the overexpression and WT plants. All those shows that the TEM1gene inhibits Arabidopsis’resistant capability under the abiotic stresses. The GFP expression experiments show that, most of the TEM1protein is distributed in the nucleus, which mainly located in nucleus.The gene TEM1plays an important role in plant growth and development process and can regulate the plant flowering time. When the gene is specifically knockout, it significantly affect the plant growth rate and results in early flowering. The TEM1mutants showed increased tolerance to abiotic stresses, indicating it played a negative regulatory role in plant abiotic stresses. |
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