PCR Assays For The Identification Of Fusarium Poae And The Prediction Model Of Its Growth

Fusarium is present in the barley in the growing, harvesting and storage process. In ninekinds of barley, the rate of Fusarium detected was low. By scanning electron microscopy ofinternal of barley it shows that, steeping barley make the surface micro-organisms washed off,but it is still wrapped in the skin. When it is at optical humidity and temperature, it began togrow.By PCR detection the reaction conditions of Fusarium poae:52℃for primer optimalannealing temperature;0.4μl Taq DNA enzyme for the optimal dosage; optimal Mg2+concentration is2.0mM; the30times of cycles. Tubulin gene primers showed goodspecificity; DNA limit of detection was417pg, and showed good agility.Use the method of predictive microbiology as growth pattern of Fusarium poae andestablish primary and secondary growth prediction model. The results show that: the primarystage model in optimal temperature curve of growth of Fusarium poae, with good effectGompertz model fitting; when close to the minimum growth temperature, suitable for thelinear regression equation; secondary growth model fit, Logistic model are slightly higherthan the Gompertz model for the correlation coefficient. Gompertz and Logistic residuals ofthe two models were within±0.05, indicating that the model growth rate forecast is accurate.With the temperature rising, the hydrophobic protein content are constantly increasing,that is, the growth of bacteria developed determines the amount of hydrophobic protein, at30℃, the hydrophobic protein content of0.31mg/ml, enough to cause gushing; Five of barleyand malt proteins (water soluble, salt solution, alcohol, alkali-soluble, foam protein) wereanalyzed. The existence of Fusarium poae changed the protein components of barley.