Biofabrication Of Patterned Bacterial Cellulose

Bacterial cellulose produced by microorganisms is the only nano-fiber material.Bacterial cellulose’s formation is carried out simultaneously with the movement ofbacterial. So the behavioral tendency of the bacteria directly determine s the ultimatestructure of the bacterial cellulose.According to the principle of the biofabrication technology, A.xylinum can grow asfunctional units on the micro-scale patterned template. Owing to the A.xylinum’soxygenotaxis and chemotactic, the bacterial cellulose fibers can realize orderlyself-assembly with this method, and the BC membrane could have specific patternedthree-dimensional network structure in the final.We marked the Acetobacter xylinum by coriphosphine O fluorescent staining andstudy their motion in a controlled patterned template microfluidic chip. Thus we can havea clear understanding on the process of production, and orderly control the assemblyprocess of bacterial cellulose fibers.Cell test proved that the micron-scale patterned surface of the bacterial cellulose ishelpful for the constraint and the induction of cells. The composites of polyaniline, carbonnanotubes and BC showed the ordered arrangement of fibers can enhance the conductivityof materials. The composites of fluorescent dye and BC showed the orderly arrangementof the fibers can improve the optical performance of the BC membrane.In short, we can produce BC that have patterned structure and ordered micro-fiberstructure on micro-nano-scale. Based on these excellent characteristics, it may have broadapplication prospects in the photoelectric materials and tissue engineering and other fields.