| Jumeau (Jumu) is the first FKH/WH protein shown to have dual function, which plays an important role in development as a typical transcription factor, and causes large-scale modification of heterochromatin possibly as a component of a multiprotein complex. Since Martin StroEdicke et al found Jumu protein in2000, the research on function of the Jumu protein is mainly concentrated on chromatin modification and development, but rarely on innate immunity.In this study, we prepared the anti-Jumu polyclonal antibody successfully, and verified the specificity of the polyclonal antibody by Western blot and immunostaining. We obtained two kinds of deletion mutant and a revertant mutant of jumu through P-element excision. In addition, we got some transgenic Drosophila strains in which jumu gene could over-express in different developmental stages and specific tissues with the use of UAS-Gal4system. The low expression and high expression mutants were used to analysis the role of jumu gene in development and differentiation of blood cell, and the result showed that the deletion of jumu gene is able to cause abnormal increase of blood cell, and the over-expression of jumu in fat body or blood cells alleviated the increase of blood cell effectively. At the same time, the result of RT-PCR detection indicated that jumu gene impact on the expression of some transcription factors associated with blood cell differentiation. Besides, the study showed that jumu gene can affect the constitutive expression of most antimicrobial peptides in larve fat body and blood cell as a positive regulator. The above discoveries provided important theoretical basis and reference value for the future study on human WHN family proteins and the role of Jumu protein in innate immune. |
PDF Full Text Request