| Objective:Through the liaoning province, jilin province and north Korea ginseng water decoction on the normal mice and spleen on the immune function of observation, compared to an immune efficacy of ginseng function, for the three places ginseng, especially liaoning ginseng market position to provide the scientific basis.Materials and methods:Kunming mice were80only, randomly divided intc blank control (AO) group, the spleen model (BO) group, three normal ginseng irrigation stomach (A1～A3) groups and three places ginseng treatment of pixu (B1～B3) group, A total of eight groups of10only, male and female half. Group B and B1～B3mice to0.4ml/only,1time/day filling the dose of take100%because senna soaking liquid10days, establish the spleen deficiency model, and other groups in the same dose irrigation under saline. Model is built, A1～A3group and B1～B3mice to press0.2ml/only/day doses were to liaoning, jilin and three north Korea ginseng decoction irrigation stomach, group A0and group B0stomach physiological saline filled to the same stimulation. Give medicine after14days materials, measurement of the spleen heavy weight and mice, and the calculation of the mice spleen index; CCK-8method to detect the spleen cells in mice activity; Flow cytometric testing mice splenic cell suspension in liquid cell apoptosis rate; ELISA test mice serum tumor necrosis factor alpha (TNF alpha) and IL-4content; HC50method to determine the content of serum hemolysin in mice.Results:1. general condition observed in miceA0～A3mice light colour, diet, behavior is normal. Ginseng decoction irrigation stomach after two weeks, A1～B3group autism, mild in mice hyperactive behavior change. B0～B3mice food, drinking water to decrease, urinate capacity is much, defecate rare pond, disorderly colour and light, sluggishness and don’t like activities, cluster. The ginseng treatment two weeks after three, B1～B3spleen deficiency symptoms of mice get better, diet water quantity and mental state basic close to normal.2. three ginseng spleen index of miceCompared with the group AO:BO group of mice spleen index significantly reduced (P<0.05); A1, A2, the A3mice spleen index no change (P>0.05), A3group had a slightly better, there were no significant differences between groups (P>0.05). B1, B2, B3group and A comparison group was not statistically significant (P>0.05).Compared with the group B0:B1, B2, B3of mice spleen index increased significantly, including B1and B2group is significant, respectively, P<0.05, P<0.01. There were no significant differences between groups (P>0.05).3.Three ginseng on the activity of mouse spleen cellsCompared with the group A0:B0of mice splenic cell activity declined obviously (P<0.05);A1, A2, the A3group no change (P>0.05), there were no significant differences between groups (P>0.05); B1, B2, B3group and A comparison group was not statistically significant (P>0.05).Compared with the group B0:B1, B2, B3of mice splenic cell activity increased significantly (P<0.05), there were no significant differences between groups (P>0.05).4. The three places ginseng of mice cell apoptosis rate affect the spleen Compared with the group A0:B0cell apoptosis rate of mice spleen significantly reduced (P<0.05); A1, A2, the A3group has increased, but this was not statistically significant (P>0.05), there were no significant differences between groups (P>0.05); B1, B2, B3group and AOcomparison group was not statistically significant (P>0.05).Compared with the group B0:the three places all ginseng can obviously increase the spleen deficiency of splenic cell apoptosis rate in mice, among them with B1, B2group of results is the most significant (P<0.05), B3group is improved, but this was not statistically significant (P>0.05). There were no significam differences between groups (P>0.05).5. The tnree places ginseng of mice serum IL-4levels of influence Compared with the group A0:BO group of mice serum IL-4content significantly reduced (P<0.01); A1, A2, the A3group raised, but this was not statistically significant (P>0.05), there were no significant differences between groups (P>0.05). B1, B2, B.3group and group AOcomparison, statistically significant difference (P<0.01).Compared with the group B0:the three places ginseng are different degrees of improvement in the serum of pixu IL-4content, to the most effective B2group (P<0.01), B1and B3group and group B is not statistically significant (P>0.05); B2and B3group has more statistically significant (P<0.05).6. The three places in mice ginseng serum hemolysin influenceCompared with the group A0:B0group of mice serum IL-4content significantly reduced (P<0.01):Al, A2, the A3group declined, but this was not statistically significant (P>0.05); There were no significant differences between groups (P>0.05). B1, B2, B3group and A0comparison group was not statistically significant (P>0.05).Compared with the group B0:B1, B2, B3group of hc50content are improved to B2and B3group effect is significant, respectively, P<0.01, P<0.05; Comparison between groups was statistically significant (P>0.05).7. The three places ginseng of mice tumor necrosis factor (TNF alpha) the influence of the contentCompared with the group A0:B0of mice TNF alpha content significantly reduced (P<0.05); A1, A2, the A3group are increased somewhat, but this was not statistically significant (P>0.05), group is also obvious difference between (P>0.05); B1, B2, B3group and A comparison group is no statistical difference (P>0.05). Compared with the group BO:B1, B2, B3mice TNF alpha content increased significantly (P<0.05, P<0.01, P<0.01), there were no significant differences between groups (P>0.05).Conclusions:1. At XiaFa relief by the spleen deficiency can lead to mice immune function is low. This experiment is the specific performance:the spleen deficiency of the spleen index model mice, and splenic cell activity and splenic cell apoptosis rate, IL-4and hemolysin content, TNF alpha’s significantly reduced.2. The three places ginseng decoction on the normal mice spleen index, the spleen cell activity, spleen cell apoptosis rate, TNF alpha content, IL-4content, the content of hemolysin were not significantly influenced.3. The three places ginseng decoction all can improve the spleen deficiency of spleen index model mice, and splenic cell activity and splenic cell apoptosis rate, And return to a normal level of basic, the three places ginseng decoction effect obvious difference; The three places ginseng decoction can improve the spleen deficiency in mice IL-4content model, the content of hemolysin, jilin ginseng is a plus. The three places ginseng decoction all can improve model rat TNF alpha content, and can make the recovery of the normal level to, the three places ginseng decoction effect obvious difference. |
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