| Purpose:The purpose of this study is to reveal the mechanism of exercise decreasing blood fat in hyperlipidemia animal models, via investigating the levels of ApoA5, FFA, VLDL and SREBP-1c. Accordingly, it conduces to provide some theorical evidences in the exercise therapy for hyperlipidemia and related complication.Methods:There were30wistar Rats which were assigned to3groups:normal diet control (ND, n=10), high fat diet sedentary control (NFD, n=10) and high fat diet exercise (NFDE, n=10), whom completed the1hour swimming exercise from Monday to Saturday every week for12weeks. After that, the levels of TG, TC, HDL-C, LDL-C, ApoA5, FFA, VLDL, SREBP-1C and Insulin were respectively determined by using enzymic method, euzymelinked immunosorbent assay and colorimetry.Results:1. There were no differences in body weight for each rat. After12weeks exercise, the body weights of the NFD rats were significantly increased as compared to the ND rats (550.52±42.22g VS.497.17±37.43g;p<0.05). However, after12weeks exercise, the body weights of NDFE rats were much heavier than these of NFD rats (482.91±.28g VS.550.52±42.22g;p<0.01).2. Compared with the ND, after12weeks, TG, TC, LDL-C, FFA, and VLDL and Insulin levels in blood serum and SREBP-1c levels in livers were significantly higher in the NFD (1.34±0.22mmol/L VS.0.61±0.21mmol/L, p<0.01;1.80±0.18mmol/L VS.1.43±0.12mmol/L, p<0.01;0.33±0.07mmol/L VS.0.15±0.03mmol/L, p<0.05;429.83±172.94μmol/L VS.220.17±74.80μmol/L, p<0.05;166.15±6.48μmol/L VS.143.40±6.19μmol/L, p<0.01).3. After12weeks, compared with ND rats, in blood serum of NFD rats ApoA5levels were lowered (43.27±6.83μg/L VS.62.72±7.62μg/L, p<0.05) and insulin levels were improved (10.91±0.50mμ/L VS.9.79±0.64mμ/L, p<0.01). In addition, SREBP-lc levels in livers were markedly increased (15.62±4.87μg/g VS.8.26±1.42μg/g, p<0.05). These indicate that high fat diet increased blood fat via the alteration of ApoA5levels, which were decreased by the up-regulation of insulin and SREBP-lc levels.4. Compared with the NFD, after12weeks exercise, TG, TC, FFA, VLDL and Insulin levels in blood serum and SREBP-1c levels in livers were significantly lower in the NFD (0.26±0.05 mmol/L VS.1.34±0.22mmol/L, p<0.01;1.32±0.11mmol/L VS.1.80±0.18mmol/L, p<0.01;275.49±101.72μ mol/L VS.429.83±172.94μ mol/L, p<0.05;140.61±15.09μmol/L VS.166.15±6.48μmol/L, p<0.01;8.65±1.56μg/g VS.15.62±4.87μg/g, p<0.01;8.70±0.64mμ/L VS.10.91±0.50mμ/L, p<0.01). The ApoA5levels in blood serum were incresed (58.84±3.42μg/L VS.43.27±6.83μg/L, p<0.05). However, there were no difference in LDL-C and HDL-C levels (0.26±0.11mmol/L VS.0.33±0.07mmol/L and0.36±0.06mmol/L VS.0.31±0.04mmol/L, P>0.05).Conclusions:1. After60minutes swimming exercise for12weeks, the body weights of high fat diet rats were improved.2. TQ TC, LDL-C, FFA and VLDL levels were significantly increased in NFD rats, and it reflects that there was hyperlipidemia in high fat diet rats and the animal models were successfully built after12weeks.3. TG, TC, FFA and VLDL levels were markedly decreased in NFDE rats after60minutes swimming exercise for12weeks, and it indicated that exercise exerted anti-hyperlipidemia effect.4. After60minutes swimming exercise for12weeks, ApoA5levels were inceased, insulin levels were decreased in blood serum, and SREBP-lc levels were lowered in livers. It showed that exercise could improve the sensitivity of insulin, increase the activity of ApoA5and down-regulated the level of SREBP-1c. For these reasons, exercise showed an important influence in anti-hyperlipidemia. |
PDF Full Text Request