Spleen Deficiency Syndrome In Rats Mtl, Aqp4, Pepsin And Proteomics Change Experimental Research

For the sea of spleen function, blood biochemical source, it is human body to the survival of warehouse degrees, so "the spleen is the root of acquired constitution", spleen deficiency, is based on a series of digestion and absorption of the comprehensive syndrome mainly obstacles, spleen qi deficiency syndrome, is the certificate of the spleen deficiency from the hair root card type, of the spleen deficiency in the modern research has been the basic theory of TCM is the important content, but has the specific diagnosis function index is few, proteomics is a comprehensive study cells, tissues and the whole life of protein inside the body and for the activities of the law science, in recent years, proteomics rapid development, has become the life science research a new field, with its strong theoretical and technical support, will be introduced to the proteomics of the spleen deficiency in the study, we will bring the research breakthrough progress. The experiments to spleen qi deficiency syndrome for model, adopt healthy group for control, this paper discusses the spleen qi deficiency syndrome happened mechanism.Purpose:This study aims to serum stomach move element (MTL), water channel protein4(AQP4), pepsin and proteomics aspects discusses the mechanism of action of spleen qi deficiency syndrome, through the observation analysis spleen qi deficiency syndrome rats MTL, AQP4, pepsin and proteomics of change, we discuss the mechanism of abnormal change happens, as the research spleen qi deficiency syndrome essence and using fill in beneficial gas decoction "in the treatment of spleen qi deficiency syndrome according to provide scientific experiments.Material and method:Choose36only SPF SD rats, the food festival (one-day feeding cabbage, double day feeding lard) add LaoJuan excessive (swimming to endurance limit) method shaping spleen qi deficiency in the rat model, giving Chinese medicine fill in beneficial gas soup, physiological saline irrigation stomach intervention. By ELISA method for determining the content of serum MTL changes, immunohistochemical method detection each rat on the difference of the water in the ileum AQP4express, than color method to determine the rats in the duodenum pepsin activity change, light-microscopy each rat stomach bowel tissue, the morphological changes, and using the method of proteomic techniques appraisal healthy controls the difference of the experimental group and protein points.Results:1. The food festival add fatigue excessive method shaping spleen qi deficiency in the rat model, made the rats appeared form gradually angular, defecate when soft dry, accidentally have loose stools, food intake, water to decrease, burnout lazy move, eyes narrowed the arch wait for a symptom; The day8to medicine, Chinese traditional medicine group spleen qi deficiency rat loose stools, lazy move, narrowed eye symptoms such as arch in ease.2. The ELISA test results serum MTL:spleen qi deficiency and the Chinese traditional medicine group of content compared with healthy MTL significantly. appear significant difference (p<0.05); Spleen qi deficiency of Chinese traditional medicine group MTL content is the obvious increase, appear significant difference (p<0.05)3. The AQP4immunohistochemical detection results in200x optical microscope. AQP4in normal expression of brown positive particles, and the amount is more; Compared with healthy controls, and the number of AQP4positive particles less, and color has a light brown; Compared with the experimental group, Chinese traditional medicine group AQP4positive particles express number significantly increased. has a deep brown.4. Pepsin testing results:the spleen qi deficiency and Chinese traditional medicine group pepsin, compared with healthy content significantly, appear significant difference (p<0.05); Spleen qi deficiency of Chinese traditional medicine group pepsin content is the obvious increase, appear significant difference (p<0.05).5. Proteomics identified a difference protein:spleen qi deficiency and healthy controls the experimental group compared with three relevant differences protein,2of them raised, a cut. Rise of the three protein respectively for glucose regulation protein78(13/A13:Hspa578kDa glucose-regulated protein), Trypsin (104/E3:Prssl Anionic Trypsin-1), the impact of a protein as albumin (20/Alb Serum albumin A20:).Conclusion:The food festival add too tired methods of shaping spleen qi deficiency in the rat model after the success, the study found that the model and the Chinese traditional medicine group rats a healthy controls MTL levels are low, AQP4positive expression is reduced, pepsin levels are low, the fill in beneficial gas soup after treatment of traditional Chinese medicine, Chinese traditional medicine group rats and the rats MTL obviously higher than content, AQP4positive expression was increased, pepsin content rise. Application of proteomics technology for identification, found that the experimental group and control group compared with three relevant differences protein,2of them raised protein for glucose regulation protein78, trypsin, a cut of the protein as albumin, these abnormal protein expression may and spleen qi deficiency syndrome happened on molecular biology mechanism.