South Banlangen Quality Evaluation Research

Baphicacanthis Cusiae Rhizoma et Radix is the dried root and rhizome of the Acanthaceae plant cusia Baphicacanthus cusia (Nees) Bremek. The stems and leaves of the Baphicacanthus cusia (Nees) Bremek.is a sources of indigo naturalis, the "Chinese Pharmacopoeia"2010edition one have been included. Baphicacanthis Cusiae Rhizoma et Radix medicinal cold and taste bitter, owned by the heart and stomach. Baphicacanthis Cusiae Rhizoma et Radix can detoxification and cooling blood away spot, commonly used in the plague, fever, sore throat, the warm poison spots, erysipelas embolism.This paper from four aspects of preliminary study on the Baphicacanthis Cusiae Rhizoma et Radix and its original plant MaLan, identify the authenticity of the goods, standardized cultivation, the cultivation of standardization,quality standards,HPLC fingerprint and so on.1. Appearance from the difference between Baphicacanthis Cusiae Rhizoma et Radix and Isatidis radix medicinal herbs on source and the characters. Carried out on two batches adulterants herbs of samples medicinal materials of the appearance characters, powder microscopic characteristics, TLC identification,and summarizes the types of common adulterants of Baphicacanthis Cusiae Rhizoma et Radix, from the plant source, roots appearance shape, TLC identification of Baphicacanthis Cusiae Rhizoma et Radix and its authenticity of the goods to be distinguished.2. In order to the traditional Chinese medicinal materials production quality management standard "for the principle, summarizes the Baphicacanthis Cusiae Rhizoma et Radix standardized planting technology, formulated the Baphicacanthis Cusiae Rhizoma et Radix standardized production procedures, and research on the Baphicacanthis Cusiae Rhizoma et Radix layering reproduction technology production influence. Comparison fo different growth years and different harvest time of Sichuan province, Baphicacanthis Cusiae Rhizoma et Radix main component indigo, indirubin contenes, determined the best harvest time and collecting fixed number of year.3. For the first time in indigo, indirubin for the indexes component, to the Baphicacanthis Cusiae Rhizoma et Radix content determination. Quality standards to the Baphicacanthis Cusiae Rhizoma et Radix microscopic identification, including cross-section and powder identification, and TLC identification based on the China pharmacopoeia2010edition of a Baphicacanthis Cusiae Rhizoma et Radix’s TLC identification method, determination of moisture, total ash, alcohol-soluble extract, the results are in line with pharmacopoeia regulation, preliminary determination of the Baphicacanthis Cusiae Rhizoma et Radix indigo content was an average of0.0088%, indirubin content was an average of0.0320%, preliminary formulated the Baphicacanthis Cusiae Rhizoma et Radix draft quality standard.4. For the first time to explore the10batches of the Baphicacanthis Cusiae Rhizoma et Radix on HPLC fingerprint by high performance liquid chromatography, screening test methods and conditions, and initial establishment the Baphicacanthis Cusiae Rhizoma et Radix medicine HPLC fingerprint common pattern, and evaluation the different origin Baphicacanthis Cusiae Rhizoma et Radix’s HPLC fingerprint similarity.