Objective:Using HBV chronic infection process different stages of the patients peripheral blood DCs and T lymphocytes of in vitro cultured cells model and traditional Chinese medicine pharmacology experiment method of plasma,comparisoning bushenjiedu and jianpijiedu which method can promote detoxification of DCs activation specific T lymphocytes, Which method is to improve the HBV infection after chronic immune cells function, immunological responses recovery, and the treatment of chronic hepatitis b main method, which for clinical improving the curative effect of TCM therapy for chronic hepatitis b provides an objective basis, treatment idea and research foundation.Method:This study by extracting HBV chronic infection immune tolerance period and immune clearance phase16patients peripheral blood80ml. Another set8cases of healthy people as control group, To cultivate a separation of mature dendritic cells, in the process of cultivation using filling kidney detoxify and spleen detoxification compound Chinese medicine drugs respectively in plasma DCs intervention, and load HBV core protein, then with own T lymphocytes trained72hours, Application flow cytometry technical inspection T cells molecular CD3, surface, CD8, CD28CD4and PD-1expression, And ELISA test kit on T cells in the fluid of qing IFN-y secretion levels. comparisoning which kind of method is intervention on the immune cells function restore much more influence.Results:1.Before the intervention of the CD4+T lymphocytes, CD28+expression level, healthy human group was significantly higher than the immune tolerance group and immune clearance group, and group of patients with immune tolerance to express the minimum(p<0.05). CD8+, PD-1expression of healthy people obviously lower than immune tolerance group and immune clearance group, which the highest expression of immune tolerance(p<0.05), CD3+results of the health and immune clearance phase groups of patients, no significant difference (p>0.05), but the resistance stage patients with low obvious group expression(p<0.05). Clear liquid secretion on IFN-γ level in health and immune clearance phase groups of patients, no significant difference (p>0.05), in tolerance period groups of patients secretion level obviously low (p<0.05).2.The immune tolerance group and immune clearance group of HBV chronic infection by the spleen and kidney patients with detoxification detoxification medicine after intervention of DC and plasma T cells of trained, testing the T cells surface of molecular expression rate. The two methods in a degree, can improve CD3+, CD4+, CD28+expression level and clear liquid on the secreted IFN-γ level, reduce the CD8+, PD-1expression level. Conclusion:1. Chronic HBV immune clearance phase and immune tolerance were present period T lymphocyte function of the defect, immune-tolerant phase in patients with cellular immune function is the lowest.2. Through the filling kidney detoxification, invigorating spleendetoxification medicine in a certain extent plasma can promote chronic HBV infection were the mature dendritic cells, making the DCs activate t-cells function improved obviously, promote T cells the expression of molecular surface, and filling kidney detoxification method to chronic HBV immune tolerance of the influence of the patient with cells immunological function is more significant. |