| Objective:To observe the change of spatial memory of rats and IL-6, IL-10expression, microglia activation condition in rat hippocampus after aerobic swimming exercise, investigate the effects of aerobic exercise on inflamm-aging and the improved effect of induced spatial memory on aging.Methods:Forty SD male rats were divided into aging exercise group (n=10), aging stilled group (n=10), control exercise group (n=10), control stilled group (n=10), aging rats were established by injection of D-Galactopyranose, the exercise group performed aerobic swimming exercise, stilled group were kept in the shallow water on the same conditions. Through the Morris water maze test,the change of spatial memory of rats were observed. The fresh cerebral tissues were divided on ice while the paraform-perfused brains were obtained The former cerebral tissues were detected by Western blot in order to investigate the changes of IL-10in hippocampus; however, the later perfused brains be done with frozen section, immunofluorescence staining, then the localization and distribution of IL-6, IL-10protein and the activation condition of Microglia were observed.Results:(1) Morris water maze:Before modeling, there are no different in the escape latency and the distance to reach the platform in four groups(P<0.05). After modeling and training, the escape latency of aging stilled group was obviously longer than that of the two control rats groups,though aging exercise group’s escape latency was short,but the difference with stilled group has no statistical significance. The aging stilled group have longer distance to reach the platform than other three groups(P<0.05). The escape latency and the distance are not different between the two control groups(P>0.05). With time increasing, place navigation showed that the escape latency and distance of each group became short. In the spatial probe, rats in each group had tendency of swimming to the quadrant which had the platform once.(2) immunofluorescence:①IL-6:the aging stilled group has more IL-6(+) neuronal cells in hippocampus CA1area than other three groups(P<0.05). The control exercise group has less IL-6(+) neuronal cells in hippocampus CAlarea than other three groups(P<0.05). There was no different between the aging exercise group and the control stilled group.②IL-10:the control exercise rats group has more IL-10(+) neuronal cells in hippocampus CAI area than other three groups(P<0.05). The control exercise rats group has more IL-10(+) neuronal cells than aging stilled group(P<0.05). The aging stilled group has less IL-10(+) neuronal cells than other three groups(P<0.05).③microglia:Observing the microglias of four groups, we found that microglias were activated in aging groups,but microglias were rested in control groups.(3) Western Blot:The aging exercise group had the highest expression level of IL-10in hippocampus CA1area in four groups(P<0.05). The control exercise group had the lowest expression level in four groups(P<0.05).Conclusion:(1)Senium could decrease the ability of learning and memory Swimming could improve the learning ability of aging rats, but it did not work in the normal rats.(2)With rats becoming old, the expression levels of inflammatory factors were increasing.(3)Aerobic exercise is a benign stress reaction of brain, it could promote expression of the anti-inflammatory factors and reduce the inflammation in the brain.(4)The inflammation in inflamm-aging may make microglial activation... |
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