Needle Foot Three Mile On Mid Model Rats Hippocampal Camp And Cgmp Level Of Influence

Objective:The multi-infarct dementia model was made by injection of emboli into the internal carotid artery. To observe the behavioral changes of MID rats, and hippocampus cAMP, cGMP content after acupuncture.Methods:1. preparation for multi-infarct dementia (MID) rat model:The multi-infarct dementia model was made by injection of emboli into the internal carotid artery. Take the same rats carotid arterial blood sampling, preparation diameter100-200μm blood clots. When application, every10mg blood clots with0.3ml normal saline made into suspension fluid. No4.5pediatric scalp needle, retrograde puncture right external carotid artery, slow injection embolus saline suspension liquid0.3ml,make embolus through the internal carotid artery intracranial arteries to the brain, cause multifocal cerebral infarction, sham operation group by the same method injection with saline0.3ml instead of embolus suspension fluid. Trials are divided into normal group,sham operation group,the model group, including control group, acupuncture group and placebo-acupuncture group.2. Acupuncture treatment:Acupuncture began after8days. Acupuncture group take double ST36acupuncture point30s.The placebo-acupuncture group take bilateral sides a fixed point (above iliac crest10mm), as a comparison exciting point, use normal reinforcement and normal reduction methods for twined technique each30s turn. Acupuncture group, placebo-acupuncture group began acupuncture after screening,1times a day, acupuncture6d, rest1d,3weeks of treatment18times. Model group, sham operation group and the normal group be gaved the same catch stimulation on time and degree with the acupuncture group and placebo-acupuncture group.3. Morris water maze hidden platform experiment:The multi-infarct dementia model was made by injection of emboli into the internal carotid artery. The dementia rats were randomly divided into3groups:model group, A normal group and a sham operation group were set up as contrast. Experiment after acupuncture. The day before the experiment, let animals swimming2min in the pool without platform, make its familiar maze environment. The experiment proceed3days altogether.The platform position is fixed and midpoint off the wall is40cm. Put the rat into water at the midpoint of quadrant with no platform, record the time from enter water to finding platform (escape incubation period), training3times every day in different entry point. With the three arithmetic means for the statistical analysis.4. After treatment, the hippocampus cAMP and cGMP contents of each group by radioimmunoassay were detected.Results:Swimming speed:The rat swimming speed has no significant difference (F=4.126, P>0.05). No significant differences between groups (F=0.854, P>0.05), each group and swimming speed does not exist between interaction (F=1.424, P>0.05).Escape latency period: in the rat escape incubation period there exist significant differences (F=6.846, P <0.05). There exist significant differences between the groups (F=12.990, P <0.05), and escape the incubation period does not exist between interaction (F=0.231, P>0.05). Compared with normal group, model group escape extended incubation period (P <0.05). Group compared with model, acupuncture group and placebo-acupuncture group escape was significantly shorten incubation period (P <0.05), but between the two groups did not see the difference obvious (P>0.05).Acupuncture influence to cAMP, cGMP content in the hippocampus: compared with normal group, cAMP content of model group decreased obviously (p <0.01). Group compared with model, acupuncture group cAMP content increased (p <0.01). Compared with normal group, model group cGMP content has not seen the abnormal changes obviously (P>0.05), acupuncture group also has not seen the obvious regulatory role (P>0.05).Conclusion:1. This experiment used injection of emboli into the internal carotid artery to make multi-infarct dementia (MID) rat model, made the observation of animals activities Bederson neurological classification rating after2days:results suggest that, the rats with symptoms and signs of MID clinical features and pathological change, model preparation success.2. Acupuncture may improve the multi-infarct dementia (MID) rat space reference memory. Possible mechanism is through regulation cAMP-PKA-CREB signaling pathways, and then to improve rats learning function and memory. Between acupuncture and placebo-acupuncture has not seen the obvious difference.3. Acupuncture may adjust the central level cAMP, cGMP imbalances state, and its mechanism may be relevant with the following approaches:activate the cAMP-PKA-CREB signaling pathways, improve inhibition to cerebrovascular expansion and cerebrovascular lasts convulsion state, reduce rat hippocampal nerve cell damage.A combination of the conclusion, Part of the mechanism that acupuncture improve multiple infarction rats dementia behavior related to regulation of the cAMP-PKA-CREB signaling pathways.