Traditional Chinese Medicine (tcm) Air Pure And Fresh And Bacteriostatic Agent Research

Air freshener of bacteriostasis of traditional Chinese medicine is based on the theory of traditional Chinese medicine, combined with modern pharmacological research, selection of Cablin Potchoull Herb, Fortune Eupatorium herb, Mint, Balsal slcullcap Root, Chinese Thoromax Root, Forsythia suspense and some other medicine, with its antibacterial components make pure preparation of traditional Chinese medicine by a certain percentage.This issue is to prove antibacterial components of Cablin Potchoull herb, Fortune Eupatorium herb, Mint, Chinese Thorowax Root, Balsal skullcap Root, Forsythia Suspensa. Then, it should choose suitable solvent preparation of antibacterial component extract, choose the best Extraction Process, select matching proposal, after primary observation of antibacterial effect, it has good effect of bacteriostasis and refreshment.First of all,To Extract volatile oil, water extract, alcohol extract of Chinese Thorowax Root, Balsal skullcap Root and Forsythia suspensa, then, to make drug sensitive slips of the same concentration of chemical, put bacteriostatic ring site as reference value, to observe smooth blood bacteriostatic effect, it concludes that antibacterial components of above6drugs are alcohol extract.Second, use aether, acetore,95%ethanol,75%ethanol,50%ethanol to reflux extraction twice of Cablin Potchoull herb, Fortune Eupatorium herb, Mint, Chinese Thorowax Root, Balsal skullcap Root, Forsythia suspensa,1h each time, merge the extract, use a rotary evaporator temperature recovering solvent and settled the volume of liquid concentration as lg/ml, prepare drug sensitive slips for smooth blood bacteriostatic effect observation, put bacteriostatic ring size as reference value, exclude solvent interference of bacterisstasis effect itself, it results that the best bacterioseatic components of6drugs are95%ethanol,95%ethanol,95%ethanol,aether,50%ethanol,95%ethanol.Third, based on the above results, it designs the best extraction of optimizing the medicine bacteriostatic component Orthogonal test. The experimental data show that, Cablin Potchoull herb use12times95%ethanol to reflux extraction3times,30min each time; Fortune Eupatorium herb use8times95%ethanol to reflux extraction twice,9min each time; Mint use12times95%ethanol to reflux extraction3time,30min each time; Chinese Thorowax Root use12times aether soaking30min, to reflux extraction3times,60min each time; Balsal skullcap Root use8time50%ethanol to reflux extraction twice,60min each time; Forsythia suspensa use10times95%ethanol to reflux extraction3times,60min each time. The liquid medicine by this extracting process on inhibition effect is greatly improved than before.Fourth, use two times dilution method of text tube for texting minimum inhibitory concentration(MIC) and minimal bactericidal concentration(MBC).Experiment results:The MIC of Cablin Potchoull herb, Fortune eupatorium herb, Mint, Chinese Thorowax Root, Balsal skullcap Root, Forsythia suspensa were:62.5mg/ml,125mg/ml,125mg/ml,125mg/ml,15.63mg/ml,3.90mg/ml; The (MBC) were:125mg/ml,250mg/ml,500mg/ml,250mg/ml,62.5mg/ml,15.63mg/ml.Fifth, orthogonal text, with bacteriostatic ring size as evaluation index, determination of best compatibility relationship of6medicine is that with the minimum inhibitory concentration for the base, high dose, low dose, low dose, low dose, high dose,high dose.Finally, Cablin Potchoull herb,Fortune Eupatorium herb, Mint, Chinese Thorowax Root, Balsal skullcap Root, Forsythia suspensa in accordance with the best technological requirements and the best compatibility relation extraction according to the ratio of mixed liquor by plate sedimentation method for indoor observation of bacteriostasis effect,it results that the air fresh bacteriotasis mixed liquid has better antibacterial effect.