Hydrogen Saturation Water Experimental Periodontitis Of Rat Anti-inflammatory Effects Of Initial Observations

Objective:Chronic periodontitis is a non-specific inflammation initiated by dental plaque microorganisms which occurres in periodontal tissues. Modern pathogenesis of periodontitis studies suggest that most of the tissue damage is due to the systemic overreaction to the pathogens. A considerable number of experiments show that oxidative stress is an important pathogenic mechanism in periodontitis. Recent studies have found that hydrogen is a novel biological antioxidants which have therapeutic effects on a variety of diseases related to oxidative stress, and have the ability to slow down periodontitis progress. This study observed the periodontal inflammation reaction in rats drinking hydrogen-rich water, and preliminarily investigated the effect of hydrogen on infectious bone disease.Methods:24 male Sprague-Dawley rats(8 weeks of age) were randomly divided into 3 groups:(1)N(the normal group):rats were used as the controls; (2)P1(the periodontitis group):periodontitis group without treatment; (3)P2(treatment group):the rats with periodontitis drinking hydrogen-rich water. In P1 and P2 groups silk ligatures were ligated around the sceond maxillary molars; the rats were fed with sucrose water and inoculated with P. gingivalis. After the model of periodontitis were built up at 21th day, the rats were sacrificed, we observed:(1)Visual assessment included tooth mobility(TM), gingival index(GI), and alveolar bone loss(ABL). (2)Histological examination (3) GSH/GSSG ratio. (4) the expression of OPG and RANKL in periodontal tissue. The collected data were statistically analyzed using variance test with the SPSS 10.0 statistics software.Results:TM(p<0.01), G1(p<0.01), ABL(p<0.05) were significantly reduced in P2 compared with P1 group. Less monocyte effusion and bone resorption lacunae were observed in P2 group. The GSH/GSSG ratio(p<0.01) and OPG expression(p<0.01) increased in P2 group; but RANKL level were not different between the two groups, OPG/RANKL ratio in P2 group was elevated relative to P1 group. Hydrogen could reduce the level of oxidative stress and protect the periodontal bone.Conclusion:Hydrogen may influence periodontal inflammatory response and reduce periodontal tissue damage and alveolar bone loss in periodontitis. The effect may be through reducing periodontal partial oxidation level and elevating OPG/RANKL rate. Therefore, Hydrogen may prevent and cure the infectious bone metabolism disease such as periodontitis..