| This thesis has a systematic study focused on the extraction and purification of polysaccharides from Limonium bicolor (Bge) Kunzt.Study on polysaccharides and trivalent iron synthetize Limonium bicolor polysaccharide-Fe(III) complex (LPC) and the synthesis LPC optimum conditions,related dynamics.physical and chemical properties and structure characterization.This paper mainly achieves the following results:(1) Limonium bicolor polysaccharide was obtained by using hot water extraction and purified to obtain higher levels of polysaccharides.The process route is:the crude polysaccharides were graded ethanol precipitation,then sevage method to remove proteins.macroporous remove the pigment,dialysis to remove oligosaccharides and inorganic salts and other impurities.Then through the gelatin column chromatographic analysis to the polysaccharide component graduation, obtains the polysaccharide content is finally60.21%purification polysaccharide (LPP),for next step carries on the polysaccharide iron synthesis to build the foundation.(2) Study on preparation of LPC reaction rules and trend by single-factor experimental.Results show that factors affecting LPC synthesis reactions are:reaction temperature,amount of solvent.the concentration of ferric chloride,pH value,adding LPP and catalyst mass ratio,in which pH value,reaction temperature,adding LPP and catalyst mass ratio play a key role.Using response surface method on three main factors influencing the synthesis of LPC to carry on the optimization of experimental. The best synthetic process conditions were obtained as follows:bath temperature of73℃,LPP/catalyst mass ratio of5:1,pH values of8,solvent volume of40ml and the concentration of ferric chloride is2mol·L-1.Ultimately,the iron content in LPC for20.48%and through experimental verification.(3) Carries on the analysis to the LPC synthesis mechanism,establishes dynamics model.The result indicated that,the polysaccharide and Fe3+has had the coordinate function, namely in the polysaccharide hydroxyl has the adsorption to Fe3+.This process explained for the available Langmuir monolayer adsorption mechanism that,also obtains the adsorption apparent activation energy is3.2213kJ/mol.(4) The physics and chemistry nature research indicated that:LPC is an iron-containing compound,good stability,soluble in water,does not contain free iron ions,their nature and iron hydroxides has a significantly different; When the relative humidity is81%and43%,the LPC hygroscopicity is better than the glycerine,more easily with water,so LPC shall be sealed and dry place to save; in the presence of a reducing agent, pH1to8range, iron ions in the LPC will restore all free in a very short period of time, similar to the human body Digest, which describes LPC can easily be restored by after ingestion, which is absorbed by the human body and has better bioavailability.(5) Through FTIR,SEM,TEM,XRD;DSC-TG and other methods to characterize the LPC, the results showed that:the LPC thermal stability is good, no obvious melting peak;micro is a dense spherical particles; the spectrum characteristic confirmed that, Fe3+and the hydroxyl of LPP has a certain degree of coordination and assumes amorphous state.The results of this study for Limonium bicolor developed into new oral iron supplement or iron food additives to provide a theoretical basis and for similar studies certain reference, with better economic, social value and prospects. |
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