Ten Red Beach To Participate In The Carbon And Nitrogen Cycle Part Of The Sandstone Type Uranium Deposit Is Culture Source Microorganism Groups Research

In nature environment, the survival and metabolic activity of microorganisms can interact with environment and thus have a major impact on the circulation of material element, migration, accumulation and deposition of mineralization in the geochemical environment. The sandstone-type uranium deposits, as one kind of the important nuclear strategic resource, is a low cost mining uranium deposits in the current technology.Objectives:In this work, in order to explore the microbial distribution rule in different ore belts and obtain the domain strain by studying the indigenous microbial revolved in carbon and nitrogen elements’circulation in xinjiang shihongtan uranium deposits.The results derived from this work will provide biological method and means for uranium deposits exploration and mining.Methods:The traditional microbiology method combained with molecular microbiology PCR-DGGE method were employed to study the saprophytic bacteria and nitrate oxidation, reducing bacteria in different geochemical ore belt of uranium ore.Results:Different types of indigenous microorganisms, like bacteria, azobacter, denitrifying bacteria, nitrobacteria and nitrite bacteria were identified in the extreme geological uranium environment. The distribution character of these microorganisms indicated that the survive and metabolic activity of microorganisms closely linked with the nature environment. The investigated uranium ore samples in the current study exhibited a certain degree of geochemical zonation characteristics:the quantities and species of bacteria was gradually reduced from the oxidation zone to the reduction zone. The bacteria were the dominant group among the saprophytic microorganisms involved in the C-element cycle. While, the proportion of actinomycetes and mold’quantity and variety was very little. In detail, pseudomonas and bacillus were the major bacteria identified in Shihongtan uranium deposits. These two types of bacteria were widely distributed due to their strong resistance characteristics. In those indigenous microorganism involved in the N-element cycle, azotobacter and denitrifying bacteria were the most exist, whereas nitrobacteria and nitrite bacteria were only exist in the oxidation zone and the redox transitional zone, these two types of bacteria demonstrated a significant geochemical zonation. Uranium content, organic carbon content and dissolved oxygen were the main factor affecting the distribution of microorganisms.The main bacteria species identified with enrichment culture method by molecular mecrobiology were Pseudomonas stutzeri strain BN15, Pseudomonas xanthomarina strain KMM1447, Pseudomonas seudoalcaligenes strain GR1, Uncultured bacterium isolate DGGE gel band Yugang-4and Shigella flexneri strain ATCC29903. The dominant groups of bacteria initially identified in the Shihongtan sandstone-type uranium was Pseudomonas.Conclusions:A variety of microbial groups exist in uranium deposits ore belt, which is the basis of mineralization effect of the microorganisms. Microorganisms can promote the circulation of the geological elements and therefore has great significance to the formation of uranium deposits.