| As development of social economics, indoor-air biological pollution has become more and more serious public health problem, an increasingly number of cases of viral infection caused by indoor-air pollution were reported. In order to providing scientific evidence for designing healthy building, the dispersion discipline of virus in indoor-air at different ventilation mode were studied in 30 cubic meters controllable ventilated biological cabin.T1 bacteriophage of Escherichia coli was used as a virus model in the study. One step growth curve of Escherichia coli:the host of T1 phage was measured. The fluctuation of T1 phage titier under different store conditions and inactivation time of the ultraviolet radiation to T1 phage were detected. Sedimentation method was used as sampling methods for quantitating the number of virus in indoor air. The distribution and dispersion of viruses in indoor air were quantitated in 30 cubic meters controllable ventilated biological cabin at 4 ventilation mode:no ventilation, up-inlet & down-outlet countercurrent ventilation, down-inlet & up-outlet countercurrent ventilation, and up/down-inlet & up/down-outlet countercurrent ventilation.One step growth curve has demonstrated that the logarithm period of Escherichia coli growth is between 1 and 4 hours. The T1 phage added equal volume glycerol stored at-20℃can keep its titer without any fluctuation. The ultraviolet radiation 30 minutes can inactivate T1 phage completely.Compared with no ventilation, up-inlet & down-outlet countercurrent ventilation, down-inlet & up-outlet countercurrent ventilation, and up/down-inlet & up/down-outlet countercurrent ventilation all speeded up dispersion of viruses in indoor air. The dispersion speed of viruses in indoor air is highest at up/down-inlet & up/down-outlet countercurrent ventilation, and second is at down-inlet & up-outlet countercurrent ventilation, Lowest is at up-inlet & down-outlet countercurrent ventilation. |
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