The Association Between Lipoprotein-associated Phospholipase A2and Ischemic Cerebrovascular Disease

Objective: Using enzyme-linked immunosorbent method to detect theplasma Lp-PLA2level of the ischemic cerebrovascular disease.To investigatethe relationship between the Lp-PLA2and ischemic cerebrovascular to predictthe occurrence of ischemic cerebrovascular disease with accurate simple andquick method.Methods:1Objects and Methods1.1Research objectswe collect the research objects from Shijiazhuang city, hebei province,and divide them into the control group and case group. The casegroup,including98cases(male62cases, female36cases, mean59.71±10.10years),is hospitalized acute ischemic cerebrovascular patients of the secondhospital of hebei medical university neurology from March,2012toFebruary,2013. the control group is the health of our physical examinationcenter in the same period, including30cases(male19cases, female11cases,mean58.82±9.27years).Gender, age matched case group and control group,research significance.1.2Exclusion criteria of the research objectAll the groups of acute ischemic cerebrovascular disease were excludedfrom the following situation:(1)bleeding after infarction;(2)cerebralhemorrhage;(3) diabetic arterial inflammatory brain infarction;(4) there is aclear source of emboli in the brain of cerebral infarction;(5)Cerebral infarctioncaused by congenital dysplasia;(6) each kind of abnormal blood coagulationmechanism and high blood viscosity caused by cerebral infarction;(7)connective tissue disease caused by cerebral infarction;(8) with coronaryartery disease, peripheral vascular disease of cerebral infarction. 1.3The grouping of the object of study(1)Focal depletion by the function defect duration of24hours or less,neurological examination no positive signs, but recurrence of hair, don’t leftthe signs and symptoms of nervous function defect after the recovery and skullMRI+DWI scan no obvious abnormal can include transient ischemic attackgroups;(2)Have focal depletion by the function defect symptom,body has nopositive signs for the nervous system，skull DWI+MRI scan have lacunarcerebral infarction, or focal ischemia but that are not the responsibility oflesions can include asymptomatic cerebral infarction group;(3)Have focaldepletion by the function defect symptom, physical examination have positivesigns of nervous system, head DWI+MRI scan can display focal cerebralinfarction of the lesions were included in the responsibility of the symptomaticcerebral infarction group.1.4Test method1.4.1Samples collection:Blood samples were taken a morning fasting venousblood in72hours, at the time of clinic visit or hospitalization in3mL serumseparator tubes by a trained phlebotomist,using EDTA as anticoagulant,centrifuged at3000g for10minutes in half an hour, and then aliquotted intoEppendorf tubes. After uniform number, samples were stored at-80°Crefrigerator until assays were run. To reduce the number of error andmeasurement error, all of the specimen are batch testing after the completion.1.4.2Detection method: Lp-PLA2mass use a Lp-pLA2quantitativediagnostic kit (microplate-based ELISA), provided by Kangerke Inc.Operation process according to the kit instructions. Other biochemicalindicators: The determination of the CHOL, TG, LDL-C, HDL-C mass usethe automatic biochemical analyzer.1.5Statistical processing:Apply SPSS13.0statistical package for statisticalprocessing. continuous variables are expressed as mean±SD. The tests ofNormality and Homogeneity of Variances use K-S test and Levene test,respectively. Continuous variables of two independent samples were assessedwith the independent-samples test. Continuous variables of multiple independent samples were assessed with the one-way ANOVA test andAmong any two samples were assessed with the SNK-q test. Nominalvariables are expressed as percentages. Nominal variables were compared byuse of the X~2test. It is a statistical significance when P＜0.05.Results:98cases of the case group include TIA group21cases, asymptomaticcerebral infarction group31cases, symptomatic cerebral infarction group46cases. Compared with control group,the of differences of the incidence ofsmoking history, drinking history, high blood pressure history, diabeteshistory had not statistically significant(P＞0.05).Compared with control group,the mass of CHOL,TG,LDL-C were higher and had statistically significant(P＜0.05) in the case group. The mass of HDL-C were lower than the controlgroup, but had not statistically significant(P＞0.05).The Lp-PLA2levels of inthe case group were significantly higher than the control group and hadstatistically significant(P＜0.05).The Lp-PLA2levels of symptomaticcerebral infarction group were significantly higher than both TIA group andasymptomatic cerebral infarction group. The differences among three grouphad statistically significant(P＜0.05).Conclusion:According to the results, the baseline characteristics of the control groupmatch with the case group. The Lp-PLA2levels of in the case group weresignificantly higher than the control group.The Lp-PLA2levels ofsymptomatic cerebral infarction group were significantly higher than both TIAgroup and asymptomatic cerebral infarction group. The Lp-PLA2levels ofasymptomatic cerebral infarction group were higher than the TIA group. Ourresults show the Lp-PLA2may be associated with the severity of ischemiccerebrovascular disease and we speculate that the Lp-PLA2possibly becomesto a biological indicators,which predict risk of the ischemic cerebrovasculardiseases.