Researches On The Clinical Value Of Prostate Cancer Diagnosis By Detecting Urine DD3by Loop-mediated Isothermal Amplification

Backgroud: PCa is one of the most common malignant tumours in old-age males.Nowadays, clinical diagnosis mostly depends on some methods, such asdigital-rectal examinantion(DRE), prostate specific antighen(PSA) and prostatebiopsy. While, digital-rectal examinantion (DRE) is relative subjective, and prostatespecific antigen (PSA) is not tiusse specificial of PCa, which causes useless prostatebiopsies and brings harm to paients, as well as the burden both on spirit and economy.Consequently, it’s a pressing matter of the moment to raise the clinical diagnosisefficiency of PCa. But traditional methods have disadvantages, such astime-consuming, complicated and high requirement for equipment, et al. So it’spressing to find out one quick, convenient and efficiencient method to raise PCadiagnosis efficiency. Loop-mediated isothermal amplification (LAMP) is a novelamplification techinque of nucleic acid. It has already been applied in gastric cancerand breast cancer, which offers technical support to improve diagnosis efficiency ofPCa by testing DD3, and shows broad prospect.Objective: By researches on the clinical value of PCa diagnosis by testing urine DD3by loop-mediated isothermal amplification, we aimed to raise the diagnosisefficiency of PCa.Method:80samples were collected from patients with abnormal serum PSA. Bypuncturing prostate biopsy,27patients were diagnosed as PCa, and53werediagnosed as BPH. TURP were operated on70paitents, and before operation30mlruine were collected after prostate chirapsia. Meanwhile, the control group of5prostate tissues and30ml urine were collected from healthy persons.Firstly, we detected DD3expression profiles of both abnormal serum PSA patientsand normal control. By comparing the results to the pathologic results, we aimed toidentify the DD3expression in PCa tissues. Then, we systemly analyzed the methodof LAMP to find out the optimize amplification conditions, such as reactiontemperature, bataine concentration and Mg2+concentration. Finally, we used LAMPwith optimized amplification conditions to detect urine DD3in both patients withabnormal serum PSA and normal control. By comparing the LAMP results topathologic diagnosis, we analyzed the clinical value of testing urine DD3by LAMP.Result: Among the80samples with abnormal serum PSA, the DD3expressionprofiles in tissues revealed all positive results in27patients, which were diagnosedas prostate cancer by prostate biopsy, and only one revealed positive expression in58BPH samples. The optimize reaction condtions of LAMP were temperature at63℃,betaine concentration as0.4M, and Mg2+concentration as4mM. The DD3expression profiles in urine revealed all positive results in27PCa patients, and onlyone positive expression in58BPH samples. The urine DD3expression profiles werenegative in5normal prostate tissues and20normal urine samples.Conclusion: The results of detecting urine DD3by LAMP were highly consistentwith clinical prostate biopsy results, which shown its clinical value in PCa diagnosis. Moreover, this novel method was simple, quick and convenient, which suggesteddetecting urine DD3by LAMP could reduce prostate biopsies, and enhance diagnosisefficiency of PCa.