Experimental Vitro Study On Triple Negative Breast Cancer Cell MDA-MB-231with Sheliugu, Curcuma Extract

Objective Observe Sheliugu and Curcuma extract accepting to triple negative breast cancer cell MDA-MB-231, as well as chemokines and adhesion molecules mRNA and protein expression changes. To provide experimental evidence for the anti-breast cancer clinical medicine.Methods Sheliugu oil ether extracts, the Sheliuguey ethyl acetate extracts, the Sheliuguey butanol extract, Sheliuguey water extract, petroleum ether extract of Curcuma Valley, the ethyl acetate extract of Curcuma Curcuma butanol extractionmaterial, aqueous extract of Curcuma intervention triple negative breast cancer lung metastasis cell line MDA-MB-231, epirubicin as a positive control saline as a negative control, CCK-8assay groups of drugs on the MDA-MB-231inhibit the proliferation of cell lines; transwell chamber migration was measured in vitro migration ability; flow cytometry assay cell cycle changes; Western Blot, RT-PCR method to detect the adhesion molecule E-cadherin, E-selectin and chemotaxisthe chemokine CCR7, SLC, SDF-1, CXCR4expression of protein and gene expression.Results①epirubicin, the snake of petroleum ether extract, high concentrations of snake the six Valley ethyl acetate extract, petroleum ether extract of Curcuma Curcuma ethyl acetate extract and high concentrations of water extract of Curcuma of MDA-MB-231cell growth were significantly inhibited petroleum ether extract of Curcuma strongest inhibition, the difference was statistically significant (P<0.05). The②pharmorubicin, snakes six Valley petroleum ether extract and aqueous extract of Curcuma petroleum ether, ethyl acetate, water extract both inhibition of MDA-MB-231cells in vitro invasion, with the saline control group compared to P<0.05, which is the most significant role of epirubicin, traditional Chinese medicine extracted species of Curcuma petroleum ether extract group most significantly, the second snake of petroleum ether extract group were statistically P between<0.05.③Analysis by flow cytometry, epirubicin and Curcuma petroleum ether, ethyl acetate could improve the in vitro growth of MDA-MB-231cells in G1phase cell ratio, reducing the S and G2cells (P<0.05);Sheliugu extract will be231cells were arrested in S phase (P<0.05), the cells can not complete DNA replication and enter the G2phase.④ether extract of Ezhu increases cell adhesion factor E-selectin expression (p<0.05), reduce the cell chemotactic factor when the CCR7, and the expression of CXCR4protein (p<0.05); Sheliugu of petroleum ether extract could significantly increase cell adhesion factor E-selectin expression (p<0.05), reduce the cell chemotactic factor when the CCR7protein expression (p<0.05); And ether extract of Ezhu role than Sheliugu of petroleum ether extract group (p<0.05).⑤ether extract of Ezhu can significantly improve cell adhesion factor E cadherin and E-selectin mRNA expression (p<0.05), reduce the cell chemotactic factor when the CCR7, SLC, and CXCR4mRNA expression (p<0.05); Sheliugu of petroleum ether extract could significantly improve cell adhesion factor E-selectin expression (p<0.05), reduce the cell chemotactic factor when the CCR7, SLC, and CXCR4mRNA expression (p<0.05); And compared with Sheliugu of petroleum ether extract, ether extract of Ezhu effect more than Sheliugu of petroleum ether extract group (p<0.05).Conclusion The results confirmed that the Sheliugu and Ezhu three negative resistance effect of breast cancer, and found that Ezhu petroleum ether extract and Sheliugu petroleum ether strongest, the MDA-MB-231cells strong inhibition may be through the increase of adhesion molecules E cadherin, E-selectin, cut chemotactic factor when the CCR7, SLC, CXCR4mRNA and protein expression.