| Objective: Gastric cancer in China is one of the most common malignanttumor, which originated in the epithelial cells of the mucous membrane of thestomach wall surface can occur in various parts of the stomach the whichAntropyloric most common, followed by gastric cardia area, the gastric bodyat least, according to the depth and breadth of the impingement of thestomach can be divided into early gastric cancer and advanced gastriccancer.If cancer limited in gastric mucosa or submucosa called early gastriccancer, such as the infringement to the muscular layer or beyond the muscularlayer or more metastatic called advanced gastric cancer. Cancer of thestomach through the naked eye can be divided into a variety of forms,including superficial type, mass type, ulcer type, infiltration type. Microscopicorganization credits included adenocarcinoma, adenosquamous Carcinoma,squamous cell carcinoma and undifferentiated carcinoma, carcinoid, in whichadenocarcinoma is the most common. Current cancer research has beendeveloped to the molecular level, normal gastric mucosa epithelial cells fromthe original (stem cells) to the growth of new cells, regardless of its growth ordeath are controlled by their own body, don’t happen out of control. Thechromosomes in stem cells includes all sorts of proto-oncogenes and tumorsuppressor genes, but in most cases proto-oncogene expression does not occur,will not cause cancer, so it will not be able to develop into gastric cancer cells.However, in some cases, such as the immune system long-term low, seriousendocrine disorders, or oncogene expression of active external adverseincentives will induce the growth of the tumor, therefore, of greatsignificance in the development of research the oncogene on research tumor.Blood vessels and lymphatic generated is process of importantphysiological of many diseases of embryonic development, tissue growth and wound healing and pathological basis. Mammals of vascular endothelialgrowth factor (VEGF, VEGF, VEGF-A-B-C, VEGF-D, VEGF-E, andplacenta growth factor and PlGF) and tyrosine kinase receptors (VEGFRVEGFR-1,2, and VEGFR-3) is one of the main angiogenic process controlsystem. In addition, these receptors play a regulatory role to the permeabilityof blood vessels and the expansion of blood vessels. Vascular endothelialgrowth factors (VEGFs) or induced lymphatic vessel formation of theimportant factors.MTA1gene is a tumor metastasis-associated gene (MTA) familymembers, because the gene was first found in breast cancer cells and relatedtumor metastasis ability, it was named for tumor metastasis-associated gene1(MTA1). The total length of2.2kb of the gene, MTA1protein havingapparent hydrophilicity, without having a transmembrane ormembrane-associated region, so it is not a cell surface or secreted protein.There are many documented MTA1protein in the signal transduction pathwayto maintain the normal function of the cell ’s protein effect, and some may beinvolved in signal transduction regulation with invasion and metastasis-related protein, thus affecting the cancer cells transfer.This experiment through the study of vascular endothelial growth factorC (VEGF-C) and tumor metastasis related gene (MTA) in human expressionand its relationship with lymph node metastasis in gastric cancer tissue so as toexplore the connection between the two.Methods:48cases of gastric carcinoma,48cases of paracancerousspecimens,20cases of normal gastric tissue sample for research， theimmunohistochemical staining method (SP method) to detect the expression ofMTA1and VEGF-C in human gastric carcinoma，and in accordance with theage of the patients with gastric cancer, gender, location, depth of invasion,and the degree of differentiation, lymph node metastasis and TNM stage on acomprehensive analysis.SPSS13.0statistical principles of statistical softwareapplications at the same time its clinical pathological type correlationanalysis. Results: MTA1positive expression in gastric cancer tissues was68.75%;positive expression rate was52.08%in the adjacent tissues, positiveexpression in normal gastric tissue was35.00%. MTA1protein expression ingastric cancer tissue was obviously higher than tissue adjacent to carcinomaand normal gastric tissue, positive expression in the tissue adjacent tocarcinoma was significantly higher than normal gastric tissue. Expressed inpatients with lymph node metastasis rate was82.76%, the expression rate was47.37%in patients without lymph node metastasis, the difference wasstatistically significant (P <0.05). Different degree of differentiation of gastriccarcinoma, the positive expression of MTA1protein is not statisticallysignificant, well-differentiated group72.73%, moderately differentiatedgroup64.29%, poorly differentiated69.57%(P>0.05). Positiveexpression of MTA1and gastric cancer tissue was positively associated withthe degree of infiltration. Carcinoma infiltrating over the serosal layer MTA1positive expression rate was81.82%, infiltration whole all intestinal wall, butnot soaked serosal layer positive expression rate was75.00%, reach themuscularis64.29%, the positive expression rate of only confined to thesubmucosa is42.86%(P <0.05). The positive expression of VEGF-C ingastric carcinoma was85.42%, in adjacent tissues, the positive expressionrate of64.58%, positive expression in normal gastric tissue was45%. Visiblepositive expression of VEGF-C in gastric cancer tissues was significantlyhigher than the adjacent tissues and normal gastric tissue. VEGF-C positiveexpression rate was94.8%in patients with lymph node metastasis in patientswithout lymph node metastasis expression rate was53.1%, the difference wasstatistically significant (P <005). No correlation of VEGF-C expression wascorrelated with the degree of differentiation of gastric carcinoma,well-differentiated81.82%,85.71%moderately differentiated, poorlydifferentiated and82.61%(P>0.05). Carcinoma infiltrating over the serosallayer VEGF-C positive expression rate was100%, infiltration of the whole allintestinal wall, but not soaked serosa93.75%, arriving myometrial71.43%,only limited to the submucosa57.14%(P <0.05), MTA1expression VEGF-C expression was positively correlated (r=0.302, P=0.037).Conclusion: MTA1protein expression in gastric cancer with tumorinfiltration depth, TNM staging and lymph node metastasis are closely related.The MTA1protein with the degree of tumor differentiation is no significantcorrelation.VEGF-C protein expression in gastric cancer with tumor infiltrationdepth, TNM staging and lymph node metastasis are closely related,VEGF-Cand tumor differentiation degree did not see obvious correlation.Of MTA1and VEGF-C gene for cancer, and the two genes may occurin the stomach, synergies exist in the process of growth, infiltration andmetastasis. Therefore, both for joint detection of gastric cancer diagnosis,treatment and prognosis judgment has very important significance. |
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