Detection Of Cd4⁺Cd25^high+CD127^low Regulator T Cells In Peripheral Blood From The Patients With Bronchiolitis And Its Significance

Bronchiolitis is one of the main caused by respiratory syncytial virus in infants is more common lower respiratory tract infections, more common in children under2years of age, small babies, especially from February to June, the disease occurs mainly in small bronchi, but also alveolarcan be involved, so it belongs to a special type of pneumonia. The disease can be found in all seasons of the year, but the incidence has certain geographical differences, time of onset of the north is mainly concentrated in the winter and spring season, but in the south the incidence also can be foung in summer and fall. A number of studies have confirmed that RSV infection, immune dysfunction exists.Studies have found that RSV bronchiolitis peripheral blood of children regulate cell (regulatory Tcell, Treg) CD4+CD25+expression level exception, but the regulatory T cells (Treg) immunological pathogenesis of RSV bronchiolitis in childrenspecific to play what role has not yet been fully elucidated, further research is needed.PurposeExplore the CD4+CD25high+CD127low regulatory T cells (regulatory Tcell, Treg) number of changes that the expression level of change in the peripheral blood of children with bronchiolitis, and further study in children with bronchiolitis immunological pathogenesis specific play what role.Materials and methodsStudy from the Third Affiliated Hospital of Zhengzhou University (Maternal and Child Health Hospital of Henan Province), the experimental design for bronchiolitis group, the bronchopneumonia group, normal control group.The specific designed as follows:1. bronchiolitis group were randomly selected treatment in our hospital from September2011to March2012,2years of age diagnosed48cases of bronchiolitis in children (28boys,20girls, the average age of11.7±2.5month), the bronchitis group of children are less than2years old the first time wheezing rule out foreign bodies and congenital abnormalities;2.25cases of bronchopneumonia bronchopneumonia group hospitalized in the same period of the same age (17boys,8girls, the average age of12.9±1.8month), the group of children were excluded from the food and drug allergies, family atopic history, a history of eczema and the use of hormones history;3.25cases of control group over the same period in hospital the same age, surgical hernia, kidney stones and other infections in children(14boys,11giris, the average age of9.4±3.2month), also excluded food and drug allergies, family atopic history, a history of eczema and the use of hormones history, in addition, the group also ruled(2weeks) in children with a history of infection and treatment. Bronchiolitis group and bronchial pneumonia, diagnostic criteria are based on Zhu Fu Tang ((The Practical Pediatrics》 diagnostic criteria.Were detected by flow cytometry in three groups of children with peripheral blood CD4+CD25high+CD127lowTreg the relative number of CD4+lymphocytes, CD4+CD25hlgh+CD127lowTreg expressed as a percentage of its expression level, and compare the three groups were detected datain order to understand the the capillary bronchial children vivo Treg cell changes. ResultsBronchiolitis in peripheral blood CD4+CD25high+CD127lowTreg in CD4+T lymphocytes (8.04±2.14)%, and bronchial pneumonia group (9.55±2.63)%relatively reduced, the difference was statistically significant (P<0.05),Compared with the control group (11.32±2.93)%lower and the difference was statistically significant (P <0.05).ConclusionChildren with bronchiolitis peripheral blood CD4+CD25high+CD1271owTreg level decreased abnormal response Treg cells may be involved bronchiolitisthe incidence and development.