| Background and ObjectiveColorectal cancer is one of the most common malignancy in the world, the incidence rate is the first3among malignant tumors of the world’s, in2006, the Ministry of Health statistics show that colorectal cancer mortality in China has been among the malignant5. Colorectal cancer and other malignancies, the cause is not yet clear, and it is related to environmental, genetic and other factors. the prevention and treatment of colorectal cancer continue to arouse people’s attention, through the detection of related genes, predicting colorectal cancer occurrence and development of therapeutic effect and prognosis is of clinical significance.Bmi-1gene belongs to a multi-gene family comb ((polycomb of genes, one of the members of the PcG) was first proposed, with the synergistic effect of the c-myc transgenic mouse lymphoma cells passaged cause cell transformation and tumor formation,and to participate in the regulation of cell proliferation, It is a recognized oncogene has confirmed that high expression of Bmi-1gene in gastric cancer, non-small cell lung cancer, nasopharyngeal cancer, breast cancer and other solid tumors were seen. Mel-18also belongs to comb gene family (the polycomb of the genes, PcG) members of the PCG involved in embryogenesis, hematopoietic cell production and cell cycle and maintain cell stability and normal cell development, Recent studies show that Mel-18is a new tumor suppressor gene in a variety of tumor tissue showed low expression or loss of expression. Bmi-1and Mel-18gene is abnormal expression in a variety of malignant tumors, but the combined detection of both expression in colorectal cancer and related research at home and abroad has so far not been reported, In this study, immunohistochemistry to detect the expression of Bmi-1and Mel-18gene in68cases of colorectal cancer and68cases of normal colorectal mucosa, to explore both the incidence of colorectal cancer development and its clinical significance.Methods1Immunohistochemistry (streptavidin-peroxidase, SP) were detected Bmi-1and Mel-18gene expression levels in68cases of colorectal cancer and68cases of colorectal normal mucosa tissue, and analysis of the level of expression of bothcancer patients, gender, age, degree of differentiation, depth of invasion, lymph node metastasis and clinical stage as well as the correlation of Bmi-1and Mel-18gene expression.2Statistical analysis Application SPSS17.0statistical software for statistical data analysis, statistical methods using the chi-square test and spearrman correlation analysis, the significance level alpha=0.05Results1. Immunohistochemical results are shown:Bmi-1gene expression in colorectal cancer, the positive rate was73.5%(50/68), in normal colorectal tissue expression was23.5%(16/68), and the difference was statistically significant(p<0.05). 2. Bmi-1gene expression in colorectal cancer tissue is closely related to cancer tissue lymph node metastasis, depth of invasion and Clinical stage (p<0.05), and the patients’gender, age and degree of tumor differentiation was no correlation (p>0.05).3. Immunohistochemical results are shown:Mel-18gene expression in colorectal cancer, the positive rate was41.2%(28/68), the positive rate was66.2%(45/68) in the normal mucosa tissues, and the difference was statistically significant (p<0.05).4. Mel-18gene expression in colorectal cancer tissue is closely related to tumor tissue lymph node metastasis, Clinical stage (p<0.05), with the patients’gender, age, degree of tumor differentiation and depth of invasion was no correlation (p>0.05).5. Statistical analysis showed that:Mel-18and Bmi-1gene expression in colorectal cancer tissue levels were negatively correlated (r=-0.545, P<0.05).Conclusions1Bmi-1of high expression and Mel-18the low expression may be associated with colorectal cancer development and metastasis.2Bmi-1and Mel-18gene expression was negatively correlated in colorectal cancer.3Combined detection of Bmi-1and Mel-18gene on the malignant biological behavior of colorectal cancer might judge significance. |
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