Expressions Of PCA-1in PCa And The Relationship Between PCA-1and The Activity Of Tumor Proliferation

Prostate cancer (PCa) is against the older men, one of the malignant tumors of the genitourinary system, the United States and Europe elderly men, the incidence is very high, showing a rising trend in recent years in the incidence of prostate cancer in China. The pathogenesis of prostate cancer and its molecular basis of development is so far unclear. With the deepening of research of prostate cancer associated molecular markers and their expression products, found many proteins associated with the development of prostate cancer and their expression products. Prostate cancer antigen fluorescent differential display analysis techniques cloned a new tumor gene, the incidence of prostate cancer is closely related to the development of. Ki-67is recognized evaluation cell proliferation activity of one of the indicators, the most widely used in the diagnosis of pathological. Ki-67positive degree can be a direct reflection of the level of activity of the cell DNA replication can be used as an important indicator to study cell proliferation. In this study, RT-PCR method PCA-1gene mRNA expression in prostate cancer research from the mRNA level; immunohistochemical technology research Ki-67expression in prostate cancer, to analyze prostate cancer PCA-1expression and tumor cell proliferation. Purpose:Explore prostate cancer tissue PCA-1expression and tumor cell proliferative activity, to provide experimental evidence for the early diagnosis and treatment of prostate cancer.Methods:First Affiliated Hospital of Zhengzhou University from January2011to May2012prostate cancer patients pathological tissue samples of153patients, aged55-82years old, with a median age of69years. According to the Gleason score standard, high, moderately differentiated carcinoma (2-7) in93cases,60cases of poorly differentiated carcinoma (8-10points); according to the WHO TNM standards, T1+T2, T3+T4of63cases90cases. Did not receive preoperative chemotherapy and hormone therapy. Prostatic hyperplasia tissue samples of135patients, aged59-80years old, with a median age of66years. All tissue samples were confirmed by histopathological diagnosis. Detection of tissue PCA-1mRNA and Ki-67antigen expression in153cases of prostate cancer and135patients with BPH tissue. Statistical analysis was performed using SPSS17。0software for data processing, a=0.05as statistically significant difference test.Results:1. In153cases of prostate cancer,138cases of PCA-1mRNA was positive expression (90.2%),135patients with BPH, nine cases of PCA-1mRNA were positive (6.7%), the difference was statistically significant (P<0.05). PCA-1mRNA expression increased with the decrease of the degree of tumor differentiation, Gleason score a score of2-7and8-10poorly differentiated group expression rates were86.0%and96.7%, the difference was statistically significance (P=0.001). PCA-1mRNA expression in PCa with increasing clinicopathological staging increase in the T1-T2and T3-T4of tumor expression rates were79.4%and97.8%, the difference was statistically significant (P=0.002).2. Ki-67protein in prostate cancer tissue expression of positive signals were mainly located in the cancer cell’s nucleus, performance nuclei were brown or yellow.135cases of prostate cancer,114cases of Ki-67protein expression was positive expression rate was74.5%,135cases of BPH tissue,24cases of Ki-67protein expression was positive expression rate was17.8%. Ki-67protein expression in prostate cancer was significantly higher than in BPH, the difference between the two was statistically significant (P<0.001). Ki-67protein expression increased with the reduction of the degree of tumor differentiation, Gleason score2-7well-differentiated and poorly differentiated group of8-10points expression rates were67.7%and85.0%, respectively, between the The difference was statistically significant (P=00.017). Ki-67protein expression in prostate cancer increased with increasing clinicopathological staging T1-T2and T3-T4in the expression rates were61.9%and83.3%, respectively, between the difference was statistically significant (P=0.003).3.153cases of prostate cancer in the PCA-1mRNA and Ki-67protein were positive for110cases,11cases were negative, PCA-1mRNA positive expression of Ki-67negative expression in28cases, PCA-1mRNA negative expression of Ki-67protein positive4cases. Both in prostate cancer was positively correlated (P<0.001).Conclusion:1. PCA-1mRNA expression and pathological stage of prostate cancer and tumor differentiation, expression was significantly higher than in advanced prostate cancer early prostate cancer, the expression in poorly differentiated prostate cancer is higher than that of well-differentiated prostate cancer. Prompt PCA-1mRNA and prostate cancer related to the degree of malignancy.2. In prostate cancer, the PCA-1mRNA expression and Ki-67protein expression was positively correlated, suggesting that PCA-1mRNA expression is possible through increased proliferative activity of tumor cells to promote malignant progression of prostate cancer.