Study On The Association Of Rs307826Polymorphisms Of VEGFR3Gene And Rs776746Polymorphisms Of CYP3A5Gene With Renal Cell Carcinoma

The incidence of RCC (renal cell carcinoma) is the result of theinteraction of environmental and genetic factors. In recent years, with thedevelopment of molecular genetic techniques, it is found that RCC may berelated with genetic polymorphisms. The relationship of geneticpolymorphisms of vascular endothelial growth factor receptor (VEGFR) geneand the cytochrome P450gene in renal cell carcinoma is gradually beingconfirmed. The purpose of this study is to explore the association of rs307826polymorphisms of VEGFR3gene and rs776746polymorphisms of CYP3A5gene with treatment of renal cell carcinoma in the south of jiangsu area.Object: To investigate the single nucleotide polymorphism of VEGFR-3(vascular endothelial growth factor receptor-3) gene and CYP3A5(cytochromeP4503A5) gene in RCC (renal cell carcinoma), find the molecular geneticmarkers that can screen high-risk groups of RCC and predict the prognosis ofRCC, and to provide the basis of prevention and individualized treatment forrenal cell carcinoma. Methods: The rs307826polymorphisms of VEGFR3gene and rs776746polymorphisms of CYP3A5gene of renal cell carcinoma was examined byPCR direct sequencing in45hospitalized patents with renal cell carcinomasurgery which are from the First Affiliated Hospital of Suzhou Universityduring August and December of2012, and then, the test results were analyzedwith the clinical data of patients.Results:1. Only1case of rs307826polymorphisms of VEGFR3showedAG heterozygous, and the rest are AA wild-type.24cases of rs776746polymorphisms of CYP3A5showed GG heterozygous,and the remaining21cases showed AG heterozygous.2. The detected genetic polymorphisms distribution of CYP3A5andVEGFR3have no significant correlations with the gender, age, tumor location,tumor pathology and tumor stage in renal cell carcinoma patients. The geneticpolymorphisms distribution of CYP3A5rs776746in right RCC more showedAG heterozygous (χ2=5.472, P=0.019).Conclusion:1. There is no obvious contact between VEGFR3geners307826SNPs and CYP3A5gene rs776746SNPs with clinical pathologyand stage of renal cell carcinoma, and there is no evidence that the two SNPscan early screen the high-risk groups of renal cell carcinoma, AGheterozygous of CYP3A5gene rs776746in right RCC is more than GG wildtype (χ2=5.472, P=0.019), but this conclusions should be confirmed by further studies.2.2%percent of VEGFR3gene rs307826SNPs is AG heterozygous inour renal cell carcinoma patients and97.8%expressed as AA wild type,andthen46.7%percent of CYPSA5gene rs776746SNPs is AG heterozygous inour renal cell carcinoma patients,53.3%showed GG wild type, this resultcould give reference for sunitinib treatment in renal cell carcinoma of south ofjiangsu area in future.3. In the south of jiangsu area, VEGFR3gene rs307826SNPs andCYP3A5gene rs776746SNPs are not the hot spot mutations in this study.