Studies On Of T Follicular Helper Cell During Schistosoma Japonicum Infection

Objective To observe the way and the mechanism of T follicular helper cellpromote the formation of granulomas during Schistosoma japonicum infection.Method Liver granulomas of C57/BL6mice after Schistosoma japonicuminfection were isolated to analyze by hematoxylin-eosin staining and cell count.Tfollicular helper cell in spleen、lymph node and liver and eosinophil in chemotaxisassays were determined by FACS respectively. Results Compared to wild typemice, after Schistosoma japonicum infection,the total number of liver granulomasfrom ICOSL-knock out mice decreased significantly and the number and ratio ofeosinophil in liver granulomas also significant decreased(P<0.05, P<0.001,P<0.05).Eosinophil express chemokine receptor CXCR4while T follicular helper cell secreteCXCL12, the ligand of CXCR4(P<0.001, P<0.001). In vitro chemotaxis assay,eosinophil migrated to the bottom well in respond to T follicular helper cell andCXCL12neutralizing antibody could effectively attenuate the chemotaxis (P<0.001).Conclusion After Schistosoma japonicum infection, liver granulomas formed inC57/BL6mice, the number of eosinophil in liver and T follicular helper cell inimmune tissues increseaed. Our results demonstrated that, by its combination withreceptors, CXCL12played a significant chemotactic role on the migration ofeosinophil. This indicates a possible role of T follicular helper cell on the migrationof eosinophil by secretion of CXCL12.