The Regulatory Role Of Infection Related Factors On Oral Lichen Planus And The Clinical Study Of Huashi Xingyu Qingre Prescription Of The Therapeutic Effect On Oral Lichen Planus

Objective：Oral lichen planus (OLP) is a chronic oral mucosal disease ofunknown etiology and long-term recurrent.The World Health Organization(WHO) defined OLP as a precancerous condition because of its tendency tocancer. In our previous research,we had selected142differentially expressedgenes and8related pathways of OLP by gene chip technology.The Epithelialcell signaling in Helicobacter pylori infection (ECS-HP) is the one of the mostimportant signaling pathway. In addition, there are some factors that areclosely related to the pathogenesis of OLP,such as infective disease-relatedfactors, immune factors and lifestyle risk factors of OLP patients.A large number of studies have shown that Helicobacter pylori (HP) isclosely related with oral mucosal infection.At present, We know that theCytotoxinassoeiatedprotein(CagA) and VaculatingcytotoxinA （VacA) aretoxins of HP,Genes encoding them are called CagA and VacA.There is noprecise reports about HP、CagA and VacA whether to play an important role inthe pathogenesis of OLP. There are specific characterization of syphilis andAIDS in oral mucosa. Whether they associated with the pathogenesis ofOLP?Many studies suggest that OLP may be a T cell mediated autoimmunediseases.C reactive protein (CRP) is an acute phase protein, it has been widelyused in the clinical detection because of its specific changes. whether theimmunoglobulin and CRP can play a supporting role in the clinical diagnosisand treatment of OLP? Scholars have increasingly focused on lifestyle riskfactors and blood type of OLP multifaceted pathogenic factors.There is noconclusion about lifestyle risk factors and blood type whether relationship ofOLP?We would detected the TP, HIV, HCV, immunoglobulin IgG, IgA, IgM,complement C3, C4, CRP, ABO blood type, HP, CagA, VacA expression in serum of the large sample OLP patients and related lifestyle riskfactorsthrough questionnaires OLP patients to analysis and explore the OLPpatients potentially pathogenic factors; Meanwhile.we detectedimmunoglobulin IgG, IgA, IgM, complement C3, C4, CRP, HP, CagA, VacAexpression in serum of OLP patients,and observed the change of clinicallesions of patients before and after treatment with Huashi Xingyu Qingreprescription,which may be provide the the experimental theoretical basis forthe future of OLP early prevention, early diagnosis and early treatment.Methods：1.Automatic chemiluminescence analysis method was used to detectedanti-TP-IgG antibody、anti-HIV-IgG antibody expression in serum of100patients with OLP.2.Enzyme-linked immunosorbent assay（ELISE）was used to detectedanti-HCV-IgG antibody expression in serum of100patients with OLP.3.Double luminosity immune turbidity analysis method was used todetected immunoglobulin IgA, IgG, IgM, complement C3, complement C4,C-reactive protein expression in serum of100patients with OLP.4.Blood type analysis method was used to detected the Blood type inserum of100patients with OLP and normal control group.To analysis therelationship between the OLP risk factors and blood type.5.Rapid urea enzyme test (RUT) was used to detected HP infectionexpression in GCF specimens of60patients with OLP.To analysis therelationship between the OLP risk factors and HP infection.6.Enzyme-linked immunosorbent assay（ELISE）was used to detected theHP, CagA, VacA expression in serum of60patients with OLP.To explore therelationship between the infection of HP、CagA、VacA and the OLP patients7.Questionnaire survey was used to60patients with OLP,To analysis andexplore the OLP patients potentially pathogenic factors and the impact on theliving conditions of the patients.8.The60OLP patients underwent Huashi Xingyu Qingre prescriptionclinical efficacy of the treatment were observed to assess the clinical effect. Results：1.The test results show that2cases anti-TP-IgG antibody-positiveexpression and0cases anti-HIV-IgG antibody-positive expression and2cases anti-HCV-IgG antibody-positive expression in serum of100patientswith OLP.Anti-TP-IgG antibody-positive patients were female. Anti-HCV-IgGantibody-positive patients were1male,1female, Anti-TP-IgGantibody,anti-HIV-IgG antibody, anti-HCV-IgG antibody no significant difference inexpression between the OLP group of patients in normal oral mucosa.2.The levels of IgG, IgM, C3, C4, CRP in the OLP patients serum wassignificantly higher than that of normal controls (P <0.05),the difference wasstatistically significant; After treatment the levels of IgG, IgM, C3, C4, CRP inthe OLP patients serum was significantly lower than before treatment (P<0.05), the difference was statistically significant.3.Blood type results showed that there is a relationship between the OLPand ABO blood type, People with blood type O are more susceptible todisease than other blood.4.HP RUT results showed that60cases of OLP patients have been tested,of which35cases of oral mucosal lesions district HP strongly positive;18cases of oral mucosal damage zone HP weakly positive;7cases showed theoral mucosal damage zone HP negative before treatment;after treatment thereare24cases of oral mucosal lesions district HP strongly positive;7cases oforal mucosal damage zone HP weakly positive;29cases showed the oralmucosal damage zone HP negative(P <0.05), the difference was statisticallysignificant.5. ELISA results show that the detection of the60cases in the serum ofpatients with oral lichen planus, the human anti HP-IgG antibodies in39cases,33cases of human anti-CagA-IgG antibody positive,31cases of human antithe VacA-IgG antibodies before treatment.After treatment the human antiHP-IgG antibodies in21cases,22cases of human anti-CagA-IgG antibodypositive,20cases of human anti the VacA-IgG antibodies (P<0.05),thedifference was statistically significant. 6.The negative life questionnaire results show that: local in centives andpsychological factors, compared with control group difference was statisticallysignificant.7.Huashi Xingyu Qingre prescription have a significant clinical effect oftreatment of OLP patients：After8weeks of treatment with Huashi XingyuQingre prescription,55cases of OLP patients with varying degrees ofimprovement，5cases of OLP patients without curative effect.The white lesions disappeared shallow healing erosions, hyperemia wassignificantly improved with Naked eye observation. Which markedly effectivein37cases (62%), effective in18cases (30%), the total efficiency of about92%, result dampness stasis Huashi Xingyu Qingre prescription prescriptionsignificant clinical effect.Conclusion：1.There is no relationship between TP、HIV、HCV virus and thepathogenesis of OLP.2.Immunoglobulin IgG, IgM, complement C3, complement C4, CRPwitha relationship of the pathogenesis of OLP. Huashi Xingyu Qingre prescriptioncan adjust the level of Immunoglobulin IgG, IgM, complement C3, C4, CRP.3.The ABO blood group with a relationship of OLP.O blood type is moreprone to OLP.4.The infection of HP and the abnormal activation of CagA, VacA wereclosely associated with OLP. Huashi Xingyu Qingre prescription can inhibitthe development of HP,CagA, VacA.5.The local incentives and psychological factors have effect with OLP.6.Huashi Xingyu Qingre prescription for OLP patients shows ansignificantly effect.