Cooperation Of Prostaglandin E2(PGE2) And Adenosine In Inhibition Of Cytotoxicity Of Tumor-infiltrating Lymphocytes In Vitro

Immune adoption treatment for cancer is one of the hotspot in cancer immune therapy. Usually, people separated the immunocompetent cells form patients for amplification in vitro, after identifying their function on killing tumor, the immunocompetent cells were back to the patients. Tumor infiltration lymphocyts(TIL) is a kind of immunocytes which were seperated form tumor tissues, it was cultured in vitro for acquiring the specific activity of killing tumor cells, with IL-2induction.The main source of it comes from surgical resection of the tumor tissues obtained by the entity and infiltration lymph nodes, and it is one of the most important methods for study and application in immune therapy currently. PGE2play an important role in development and multiplication of lymphocytes, and adenosine is recognized as a molecule for inhibiting the immune function of T lymphocyts. Both of them are involved in immunoregulation, but their influence on the function of TIL has been reported little. Thus we designed a experiment to explore the exactly function of the two molecules on TIL. In our research, the effect of PGE2and adenosine on the cytotoxic activity and cytokine production of TIL from patients with stomach cancer was investigated. We found that PGE2and2-chloroadenosine(CADO; a stable analog of adenosine) directly inhibit the cytokine production and cytolytic function of TIL, and the combination of them have additive suppressive effects. Thus we believe that synergic effects of PGE2and adenosine can inhibit the activity of TILs.