| The radiation damage to human are highly dependent on the energy distributionand a series biological and chemical process in body. Once DNA was established as themolecule that encodes the genetic instructions used in the development and functioningof all known living organism and many viruses, it was suggested as the critical target.This was the start of the target theory, which states that there is a critical point in the cellthat must be hit in order to induce cell death, and radiation damage outside of this targetshould not cause cell death. For many years it has been thought that DNA is the criticaltarget in a cell exposed to radiation, and although much evidence has accumulated tosuggest that there are other targets within the cell that can lead to significant damage,DNA is still of critical importance. Cell is the basic unit of human body and the innerenvironment of cell has influences on radiation effect. Thus, it’s necessary to study theradiation mechanism on cell level.This paper is to establish a method to modeling a single cell, which can be appliedin Monte Carlo simulation.Materials and Methods: KB cell, as being typically tumor cells, is chosen as ourresearch object. After the cells are cultured and stained, images are taken by confocalmicroscopy. Data is extracted from images and imported into Geant4for modeling.Mean specific energy in nucleus and cytoplasm is calculated separately.Results:7single cells are analyzed. Considering calculating time and accuracy,the resolution scales are set in the range of0.3-0.4μm and the number of αparticle with3MeV energy is set to be200.The calculating results shows that by a single particle hit,the mean specific energy in nucleus is0.209Gy and that in cytoplasm is0.044Gy.Conclusion: the modeling method we developed is practical and universal, inwhich the density of nucleus and cell shape effect the calculating results a lot. This study is based on open-source software and the results are in agreement withpublished data. |
PDF Full Text Request