The Preliminary Study Of COX-2Inhibitor Celecoxib’s Radiosensitizing Effects On Glial Cell OLN93and Glioma Cell U373

Objective:To evaluate Celecoxib’s radiosensitizing effects on glial cell OLN93and glioma cellU373by contrast the different effect of Celecoxib on normal brain glial cells and gliomacells and to explore its mechanism of action.Methods:This paper Choose normal glial cell OLN93and human brain glioma cell U373asresearch subjects, and sets two drug groups (group D, group D’) according to differentconcentrations of Celecoxib (0μM,10μM,20μM,40μM,60μM,80μM), and sets anothertwo radiation groups (group R, group R’) according to different dose of6MV-X-rayirradiation (0Gy,0.5Gy,1Gy,2Gy,4Gy,6Gy,8Gy) as well. After that, drug and radiationgroups (group D+R, group D’+R’) are also established. we observed the cell’smorphological reaction under the influence of Celecoxib, Then, we individually conductMTT experiment to measure growth inhibition rate of cells, cell scratch experiment todetect migration ability of cells, cell colony formation to draw cells survival curves andflow cytometric technology to detect cells cycle distribution.Results:1. The result of cells morphology: the morphology of glial cell OLN93and gliomacell U373were changed after24hours with adding Celecoxib of different concentrations.Meanwhile, the higher the concentration of drugs added, the more obvious the reaction wasobserved.2. The outcome of MTT experiment: Celecoxib could inhibit the growth of bothOLN93cell and U373cell in a dose-dependent manner, and the inhibition effect on U373 cell was more obvious.3. The outcome of cell scratch experiment: The migration ability of both OLN93celland U373cell became weakened with the effect of Celecoxib of40μM, and the inhibitioneffect to U373cell was more obvious.4. The outcome of cell colony formation: Celecoxib of40μM couldn’t increase theradiation sensitivity of OLN93cell whose SER=1.04; but could increase the radiationsensitivity of U373cell whose SER=1.27.5. The outcome of Flow cytometric analysis: Celecoxib of40μM didn’t affect the cellcycle distribution of OLN93cell, but increased U373cell’s proportion in G2/M phase;Ray could increase OLN93cell and U373cell’s proportion in G2/M phase; Celecoxibcouldn’t induce Ray to increase OLN93cell’s proportion in G2/M phase, but could induceRay to increase U373cell’s proportion in G2/M phase.Conclusions:1. Selective COX-2inhibitor Celecoxib could inhibit the growth of normal glial cellOLN93and glioma cell U373as well as their ability of migration to a certain extent.2. Celecoxib couldn’t enhance radiosensitivity of normal glial cell OLN93, but has aradiosensitization effect on glioma cell U373.3. Celecoxib regulates glioma cell cycle distribution and increases cell proportion inG2/M phase, which maybe one of the mechanisms of enhancing radiosensitivity.4. Celecoxib, a COX-2selected inhibitor, could be widely clinical used as an effectedradiosensitizer.