Pathological Analysis Of Body Surface Tumors Growth Range

Objective:This article is to observe morphological characteristics of the body surfacetumor in different layers by making whole-mount serial and selective sections for isolatedtumor tissue specimens of patients with body surface tumor; whole-mount serial sectionsare to be performed a pathological three-dimensional reconstruction by digital section scanand analysis system and3D view; under the guidance of principles of stereology, thisarticle is to summarize three-dimensional morphological characteristics of body surfacetumors of different types at different periods and their relationships with peripheral normaltissue infiltrations, and to discuss the confirmed standard for the personalized excisionextension of the tumor, giving directions to the full excision of tumor tissues and theimprovement of precise tumor excision during surgery clinically.Methods: A full and extensive resection was made on patients diagnosed with bodysurface tumor. For single lesions, their isolated tumor mass specimens were wholly fixed informalin and were made whole-mount serial or selective sections and performed HE stainsafter gradient dehydration with alcohol and paraffin embedding. Whole-mount serialsections were scanned by digital pathological image scanner and the relationship betweentumor cell and normal tissue was observed on the digital image through the display; therange of tumor cells was marked with a mouse, and digital section virtual images scannedwere performed a pathological three-dimensional reconstruction through thethree-dimensional reconstruction software to observe morphological characteristics of thetumor. Whole-mount selective sections were observed the relationship between tumortissue and normal tissue infiltration as well as the pattern and feature of tumor growthunder the fibrescope. For sporadic lesions, according to the principle of stereology, thetumor specimens of the diagnosed scrotal Paget’s patients were marked24points at0.5cm,1.5cm and2.5cm away from visual margin of tumor along with the direction of12,1.5,3,4.5,6,7.5,9,10.5o’clock, then the small piece of tissues at these24points were removed in each patient for HE staining and routine pathological diagnosis. According to thedistance from the point that firstly presented negative result to the visual margin of tumor,the relationship between the visual margin of tumor with different diameters and thepathological diagnosis margin was concluded.Results:By observing pathological three-dimensional reconstruction images of the tumor,it could be found that the tumor tissue of basal cell carcinoma less than2cm wassignificantly asymmetric, well-defined with peripheral tissues, smooth-base, with“pan-bottom-like” growthform, and that it grew rarely “pseudopodia”, whose lengths wereless than deep fascia. The range of resection can be expanded to1.5cm if the tumordiameter is smaller than or equal to10.9cm; the range of resection need to be expanded tomore than2.5cm if the tumor diameter is larger than or equal to10.9cm.Conclusion: Whole-mount sections are significant to the research on body surface tumor.Both digital pathological section scanning technology and pathological sectionthree-dimensional imaging technology developed based on this provide not only a morescientific way to study the tumor morphology but a new and reliable way to observe thegrowth pattern of the tumor morphology. Different types of tumors in different periodsshow different patterns of peripheral normal tissue infiltrations; precise excision extensionof the tumor should be determined individually and obtained by observing, analyzing andsummarizing plenty of pathological three-dimensional reconstruction images of the tumor.Under the guidance of principles of stereology,With the increase in the tumor’s diameter,the distance between the visual margin and the pathological margin need be expandedmore distantly for the determination of surgical reaction range.