The Effect Of Deformable Embedding Medicated Injection Thread In Acupoints On Brain Tissue PCNA And Caspase-3of Rats With Focal Cerebral Ischemia Reperfusion Injury

ObjectivesThe project to tetramethylpyrazine as a model drug, the use ofa biodegradable polymer material, The effect of Ligustrazineprepared into suitable transformation of acupoint injection therapy,it has at room temperature for the liquid, semi-solid gel is formedin the bodyshape. Selected cerebral ischemia and reperfusion rat,the its Dazhui and the bilateral Neiguan acupoints is burieddeformable embedding medicated injection thread treatment, and acomparison with the Ligustrazine collagen medicine line group andLigustrazine medicinal catgut. Methods to observe the treatmentusing immunohistochemistry after cerebral cortex of ratproliferating cell nuclear antigen (PCNA) and cysteine asparticproteinase-3(Caspase-3) regulationMethodsReference experimental research in the study, the use oforthogonal design method, the chitosan mixture transformationacupoint prepared by mixing anhydrous of dipotassium hydrogen andtetramethylpyrazine injection drug line, And the medicine on the wireinto the rabbit after acupuncture points, influence on the releasedegree of its body and the medicine line on cerebral cortex in ratsPCNA, Caspase-3. 1.The transformation point injection drug lines in vivo drugrelease test: the choice of healthy rabbits, the thetetramethylpyrazine drug catgut tetramethylpyrazine collagen druglines and can be transferred to the form of drug catgut buried bya certain dose rabbits NeiguanDazhui, Using high performance liquidchromatography (HPLC), plasma concentration in rabbits in differentperiod, comparing the three kinds of medicine line, assessed the invivo release properties of drug line.2. In terms of effects research on brain tissue PCNA and Caspase-3of rats with focal cerebral ischemia reperfusion injury：40healthySD rats were randomly divided into4groups, respectively, modelgroup transformation point injection drug line group, thetetramethylpyrazine pharmaceutical catgut group and ligustrazinecollagen medicine line group, four groups of10mouse. Improved lineintraluminal focal cerebral ischemia reperfusion model (MCAO) inrats Ojo, bilaterally on the embedding of drug line treatment.24hafter the last observation date using cardiac perfusion technologydecapitation the brain was fixed in4%paraformaldehyde solution of6-8h, embedded in paraffin, with immunohistochemical methoddetermination of rat brain cortex of PCNA, Caspase-3expression, andhigh-resolution color image analyzer HPIAS-1000computer imageanalysis, the data obtained were analyzed statistically sections.Results⑴The transformation point injection drug lines in vivo drugrelease test: HPLC method, determination of rabbit plasmaconcentrations of drugs catgut group reached the maximum after28h,then decreased gradually; collagen drug line group peaked at24hafter release value, followed by a gradual decline; turn shaped drug line group22h after release value is the highest value, followedby a gradual decline, still release the expression32h after.⑵Immunohistochemical detection of the experimental results showthat: the model group rat brain cortex Caspase-3positive cellsincreased significantly, there is a small amount of PCNA expression,transformation acupoint injection drug line group, thetetramethylpyrazine drug catgut group tetramethylpyrazine collagendrug linegroup compared with the model group at the same time, thereare significant differences which can be transferred to the form ofthe acupoint injection drug line group by Caspase-3increased PCNAprotein expression (P <0.05); obvious effect of Caspase-3, PCNAexpression excellentin the latter two groups (P <0.05); latter twogroups were also compared, no significant difference (P>0.05).Conclusions1. Transformation point injection drug release in vivo in ratsin vivo release time to teach slow, have a good slow-release effect.2.transformation point injection drug can upregulate of PCNA downCaspase-3, Caspase-3reducing damage to the cerebral cortex, reducecerebral ischemic injury. The transformation point injection drugline of PCNA, Caspase-3affect significantly better than thetetramethylpyrazine drugs catgut and of tetramethylpyrazinecollagen drug line, which is closely related with the turning-pointinjection drug line slow release characteristics of the drug.