Relationship Of ALDH2and MFN2Polymorpifsms With Coronary Heart Disease In Chinese Han Population Of Northern Anhui Region

Objective:To investigating the distribution between rs873457variant of mitofusin2(Mfn2) gene, RS671variant of aldehyde dehydrogenase2(ALDH2) gene polymorphisms and This study is designed to assess whether genetic polymorphisms in mitofusin2(Mfn2) gene, aldehyde dehydrogenase2(ALDH2) gene is associated with CHD susceptibility by a case-control study and the possible mechanisms initially.Methods:A case-control study method was adopted320patients with CHD and136healthy volunteers in the northern areas were included the basic data collected containing the subjects clinical characteristics, including gender, age, drinking, smoking, hypercholesterolemia. Genomic DNA was extracted from anticoagulant whole blood Genotyping were determined using the PCR-RFLP and identified by sequencing.One-way ANOVA.T-test and Chi-square(X2),Logistic regression were performed by use of SPSS17.0software.Result:1.(1)The genotype distribution of ALDH2rs671gene polymorphism are consistent with Hardy-Weinberg equilibrium law.(2)The genotype frequency of rs671polymorphism of the ALDH2gene exists G, A the two alleles and GG, GA, AA the three genotypes. The A allele frequency43.0%of rs671polymorphism of the ALDH2gene CHD group is significantly higher than the frequency34.6%in healthy control group. Significant difference was observed between two groups(P<0.05).(3)Further study showed that there were significant differences in levels of systolic blood pressure, pulse pressure, fasting glucose, serum insulin levels, CRP, HDL, Alcohol consumption among different genotypes of ALDH2gene in CHD patients(p<0.05),Patients with AA/GA genotype of ALDH2had bigger systolic blood pressure,pulse pressure,fasting glucose,serum insulin levels,CRP, Alcohol consumption than that in GG genotypes and smaller HDL than that in GG genotypes.Logistic regression analysis showed that the AA genotype was an independent effect factor for CHD (OR=2.552,95%CI:1.256-5.185).inclusion of Alcohol consumption,CRP,HDL as three of the independent variables downplayed the importance of the ALDH2genotype (OR=3.225,95%CI: 1.597-6.510).2.(1)The genotype distribution of Mfn2rs873457gene polymorphism are consistent with Hardy-Weinberg equilibrium law.(2) The genotype frequency of rs671polymorphism of the Mfn2gene exists G,C the two alleles and GG, GC, CC the three genotypes. The A allele frequency47.5%of rs873457polymorphism of the Mfn2gene CHD group is significantly higher than the frequency39.3%in healthy control group. Significant difference was observed between two groups(P<0.05).(3) Further study showed that there were significant differences in levels of systolic blood pressure,diastolic blood pressure,pulse pressure,fasting glucose,serum insulin levels,CRP among different genotypes of Mfn2gene in CHD patients(p<0.05),but age, gender, drinking, smoking,diabetes history of the two group was not significant.(p>0.05)..Logistic regression analysis showed that the CC genotype was an independent effect factor for CHD (P=0.027, OR=1.581,95%CI:1.052-2.375), inclusion of blood pressure, HOMA-IR, CRP as three of the independent variables downplayed the importance of the ALDH2genotype(P=0.016, OR=1.739,95%CI:1.110-2.724).Conclusion:The rs671polymorphism of ALDH2is significantly associated with CHD in Chinese Han Population of Northern Anhui Region. ALDH2genotypes affects on CHD by independent mechanism ranther than by alcohol consumption.and allele A may be the independent risk factor of CHD.The rs873457polymorphism of mfn2was significantly associated with CHD,and allele G was the risk factor of CHD. The allelic variation of the Mfn2gene locus in conjunction with blood pressure, insulin resistance index, CRP maybe three factor contributing to coronary heart disease and increase the risk of CHD.