Effects Of IL-2on Proliferation And Apoptosis Of MG63under High Glucose Cultivation

Objective and background:Because of the increasing attention of oral health, the type ofpatients is growing gradually in clinical. The most difficult problem is theserious alveolar bone resorption after a long time agomphosis. Both of theheight and width defection bring a difficult problem for the Clinicaldental restorations. And the kind of patients, who has many years historyof diabetes, the lack of the alveolar bone resorption is more serious thanthe normal person. This phenomenon makes the denture restorationundesirability, and the quality of the life is hard to improvement.One of the most common complication of diabetic patients isosteoporosis. As part of the body, the alveolar bone is affected naturally.The blood glucose of diabetic patients is keeping a high level for a longtime, and the high blood glucose is the initiating factors of diabeticosteoporosis.So-called osteoporosis, is the loss of per unit volume bone associatedwith the decreased of bone strength. It is mainly about the damage ofdynamic balance between bone resorption and bone formation. Boneformation and bone resorption is completed by osteoblasts and osteoclastsrespectively, and the number and the activity of the osteoblast affect thequality of bone. The proliferation and activity of osteoblast is influencedby many factors, including hormone, local growth factors and cytokines,etc. This experiment through culturing MG63cells in vitro with highsugar medium effect on cells, and simulating the high blood glucoseenvironment of diabetic patients. Culture osteoblasts with different concentrations of interleukin-2as drug intervention in sugar medium.Observe the proliferation, cell cycle and apoptosis of osteogenesis cellswith different concentrations of interleukin-2. Discussion the role andmechanism of interleukin-2as intervention drugs, which provide thetheoretical reference for future clinical trials and related basic research.Methods:Recovery the MG63cell lines, and vaccination in25ml cell culturebottle, placed in the cell culture incubator, vaccination in96-well platesand six orifice respectively plate after subculture. Experiment is dividedinto five groups:①Blank control group: normal medium;②The highsugar group: high sugar medium;③Interleukin-2intervention group1:high sugar medium+IL-2(100u/ml);④Interleukin-2interventiongroup2: high sugar medium+IL-2(500u/ml) training;⑤Interleukin-2intervention group3:sugar medium+IL-2(1000u/ml).5samples of eachgroup in96-well plates, one sample of each group in6orifice, repeat3times. Detection the MTT value of the control group and experimentalgroup in48h, detection the cell proliferation and apoptosis by flowcytometry instrument, and statistical analysis.Results:①The MTT method determined that the high sugar group cells islower than the blank control group; IL-2intervention group is higher thanthe high sugar group, but still lower than the blank control group.②Cell cycle analysis by flow cytometry instrument, obtain that highsugar restrain osteoblast proliferation. After the interleukin-2intervention,the inhibition of osteoblast proliferation is eased, and with the increasingof interleukin-2’s concentration, the relaxation effect is also increasesubsequently. ③Cell apoptosis rate by flow cytometry instrument, obtain thatosteoblast apoptosis rate was significantly higher than the blank controlgroup under the culture of high sugar medium, and the Interleukin-2intervention group is obviously lower than the high sugar group, and withthe increasing of interleukin-2’s concentration, the apoptosis rate isdecreased subsequently.Conclusion:1. High sugar cultivation can promote osteoblast apoptosis;2.Interleukin-2by high sugar cultivation can inhibit the cellproliferation;3.With the increasing of interleukin-2’s concentration, the inhibitionalso showed a growth trend.